Antitumour activity of Salmonella typhimurium VNP20047 in B16(F10) murine melanoma model.

• Authors: Joanna Jazowiecka-Rakus , Stanisław Szala
• Languages of publication: EN

Abstracts

EN

A tumour therapy is proposed based on attenuated Salmonella typhimurium VNP20047 expressing the Escherichia coli cytosine deaminase gene. VNP20047 was administered intravenously to B16(F10) melanoma-bearing C57BL/6 mice. VNP20047 proliferated within tumours and livers regardless of the initial inoculum dose. After 10 days the number of bacteria increased in livers up to 4.2 × 106 cfu/g and decreased in tumours down to 5.9 × 106 cfu/g. VNP20047 at 1 × 105 cfu/mouse, when combined with 5-fluorocytosine, inhibited tumour growth by 85% without prolonging animal survival. Histology studies revealed severe lesions in tumours and livers. These data suggest that S. typhimurium VNP20047 induced inflammatory responses, even though the strain was attenuated.

Keywords

EN

cancer gene therapy; tumour targeting; cytosine deaminase; Salmonella

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 3
• Pages: 851-856

Dates

published

2004

received

2004-03-02

revised

2004-04-23

accepted

2004-04-27

Contributors

author

Joanna Jazowiecka-Rakus

• Department of Molecular Biology, Centre of Oncology, Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland

author

Stanisław Szala

• Department of Molecular Biology, Centre of Oncology, Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland

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