The mystery of reactive oxygen species derived from cell respiration.

• Authors: Hans Nohl , Lars Gille , Katrin Staniek
• Languages of publication: EN

Abstracts

EN

Mitochondrial respiration is considered to provide reactive oxygen species (ROS) as byproduct of regular electron transfer. Objections were raised since results obtained with isolated mitochondria are commonly transferred to activities of mitochondria in the living cell. High electrogenic membrane potential was reported to trigger formation of mitochondrial ROS involving complex I and III. Suggested bioenergetic parameters, starting ROS formation, widely change with the isolation mode. ROS detection systems generally applied may be misleading due to possible interactions with membrane constituents or electron carriers. Avoiding these problems no conditions reported to transform mitochondrial respiration to a radical source were confirmed. However, changing the physical membrane state affected the highly susceptible interaction of the ubiquinol/bc1 redox complex such that ROS formation became possible.

Keywords

EN

hydrogen peroxide; reactive oxygen species; mitochondria; phospholipid membrane; superoxide; bc1 complex

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 1
• Pages: 223-229

Dates

published

2004

received

2003-11-10

revised

2004-01-12

accepted

2004-01-13

Contributors

author

Hans Nohl

• Basic Research in Pharmacology and Toxicology, University of Veterinary Medicine Vienna, Vienna, Austria

author

Lars Gille

• Basic Research in Pharmacology and Toxicology, University of Veterinary Medicine Vienna, Vienna, Austria

author

Katrin Staniek

• Basic Research in Pharmacology and Toxicology, University of Veterinary Medicine Vienna, Vienna, Austria

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