Application of electrophoretic methods for detection of protein-porphyrin complexes.

• Authors: Agnieszka Kowalska , Piotr Kwiek , Ewa Miłosz , Grzegorz Gondek , Anna Romiszewska , Alfreda Graczyk , Anna J Podhajska
• Languages of publication: EN

Abstracts

EN

Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel DocTM 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-transferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins.

Keywords

EN

porphyrins; photodynamic therapy; PDT; electrophoresis

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 4
• Pages: 1155-1163

Dates

published

2003

received

2003-10-13

revised

2003-11-26

accepted

2003-12-09

Contributors

author

Agnieszka Kowalska

• Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland

author

Piotr Kwiek

• Department of Acousto-optics and Laser Physics, Institute of Experimental Physics, Faculty of Mathematics, Physics and Informatics, University of Gdańsk, Gdańsk, Poland

author

Ewa Miłosz

• Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland

author

Grzegorz Gondek

• Department of Acousto-optics and Laser Physics, Institute of Experimental Physics, Faculty of Mathematics, Physics and Informatics, University of Gdańsk, Gdańsk, Poland

author

Anna Romiszewska

• Institute of Optoelectronics, Military University of Technology, Warszawa, Poland

author

Alfreda Graczyk

• Institute of Optoelectronics, Military University of Technology, Warszawa, Poland

author

Anna J Podhajska

• Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland

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