Cytokine action and oxidative stress response in differentiated neuroblastoma SH-SY5Y cells.

• Authors: Joanna Kania , Aleksandra Barańska , Amalia Guzdek
• Languages of publication: EN

Abstracts

EN

In the retinoic acid-differentiated neuroblastoma SH-SY5Y cells, IL-1 induced binding activity of NFκB and up-regulated the expression and activity of MnSOD. The IL-1-elicited effects were partly reversed by IL-4 and IL-6. It is proposed that IL-4 and IL-6 may participate in the regulation of the imbalanced oxidant status induced by IL-1 in differentiated neuroblastoma cells. In the SH-SY5Y cell line, TNFα neither activated NFκ nor induced MnSOD expression and activity, but was capable of modulating the IL-1 effects. Pyrrolidine dithiocarbamate (PDTC), an inhibitor of NFκB activation, down-regulated the expression and activity of MnSOD, which may suggest that the regulation of MnSOD by IL-1 in retinoic acid-differentiated neuroblastoma cells was mediated by the nuclear factor κB.

Keywords

EN

neuroblastoma cells; NFkB; cytokines; PDTC; MnSOD

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 3
• Pages: 659-666

Dates

published

2003

received

2003-05-30

revised

2003-08-11

accepted

2003-09-10

Contributors

author

Joanna Kania

• Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, Kraków, Poland

author

Aleksandra Barańska

• Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, Kraków, Poland

author

Amalia Guzdek

• Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, Kraków, Poland

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