Ca2+differently affects hydrophobic properties of guanylyl cyclase-activating proteins (GCAPs) and recoverin.

• Authors: Wojciech A Gorczyca , Marcin Kobiałka , Marianna Kuropatwa , Ewa Kurowska
• Languages of publication: EN

Abstracts

EN

Guanylyl cyclase-activating proteins (GCAPs) and recoverin are retina-specific Ca2+-binding proteins involved in phototransduction. We provide here evidence that in spite of structural similarities GCAPs and recoverin differently change their overall hydrophobic properties in response to Ca2+. Using native bovine GCAP1, GCAP2 and recoverin we show that: i) the Ca2+-dependent binding of recoverin to Phenyl-Sepharose is distinct from such interactions of GCAPs; ii) fluorescence intensity of 1-anilinonaphthalene-8-sulfonate (ANS) is markedly higher at high [Ca2+]gfree (10 μM) than at low [Ca2+]free (10 nM) in the presence of recoverin, while an opposing effect is observed in the presence of GCAPs; iii) fluorescence resonance energy transfer from tryptophane residues to ANS is more efficient at high [Ca2+]free in recoverin and at low [Ca2+]free in GCAP2. Such different changes of hydrophobicity evoked by Ca2+ appear to be the precondition for possible mechanisms by which GCAPs and recoverin control the activities of their target enzymes.

Keywords

EN

fluorescence; calcium-binding proteins; recoverin; guanylyl cyclase-activating proteins

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 2
• Pages: 367-376

Dates

published

2003

received

2003-04-28

revised

2003-05-30

accepted

2003-06-07

Contributors

author

Wojciech A Gorczyca

• Laboratory of Signaling Proteins, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland

author

Marcin Kobiałka

• Laboratory of Signaling Proteins, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland

author

Marianna Kuropatwa

• Laboratory of Signaling Proteins, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland

author

Ewa Kurowska

• Laboratory of Signaling Proteins, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland

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