Methods of peptide conformation studies.

• Authors: Andrzej Biedrzyński
• Languages of publication: EN

Abstracts

EN

In solution most of the peptides assume multiple flexible conformations. Determination of the dominant conformers and evaluation of their populations is the aim of peptide conformation studies, in which theoretical and experimental methods play complementary roles. Molecular dynamics or Monte Carlo methods are quite effective in searching the conformational space accessible to a peptide but they are not able to estimate, precisely enough, the populations of various conformations. Therefore, they must be supplemented by experimental data. In this paper, a short review of the experimental methods, most widely used in peptide conformational studies, is presented. Among them NMR plays the leading role. Valuable information is also obtained from hydrogen exchange, fluorescence resonance energy transfer, and circular dichroism measurements. The advantages and shortcomings of these methods are discussed.

Keywords

EN

fluorescence resonance energy transfer; NMR; circular dichroism; peptide conformation; hydrogen exchange

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 4
• Pages: 1091-1099

Dates

published

2001

received

2001-10-24

accepted

2001-11-14

Contributors

author

Andrzej Biedrzyński

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, A. Pawińskiego 5A, 02-106 Warszawa, Poland

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