Inhibition of the helicase activity of HCV NTPase/helicase by 1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide-5'-triphosphate (ribavirin-TP).

• Authors: Peter Borowski , Melanie Lang , Andreas Niebuhr , Annemarie Haag , Herbert Schmitz , Julian Schulze zur Wiesch , Joonho Choe , Maria A Siwecka , Tadeusz Kulikowski
• Languages of publication: EN

Abstracts

EN

In the presented study the ribavirin-TP - an established inhibitor of the NTPase activity of the superfamily NTPase/helicases II - was investigated as an inhibitor of the unwinding activity of the hepatitis C virus (HCV) NTPase/helicase. The kinetics of the reaction revealed that ribavirin-TP reduces the turnover number of the helicase reaction by a mechanism that does not correspond to that of the inhibition of the NTPase activity. Our results suggest that derivatives of ribavirin-TP with enhanced stability towards hydrolytic attack may be effective inhibitors of the enzyme.

Keywords

EN

ribavirin-5'-triphosphate; enzyme inhibition; HCV helicase

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 3
• Pages: 739-744

Dates

published

2001

received

2001-01-8

revised

2001-04-4

accepted

2001-07-4

Contributors

author

Peter Borowski

• Abteilung für Virologie, Bernhard-Nocht-Institut für Tropenmedizin, Hamburg, Germany

author

Melanie Lang

• Abteilung für Virologie, Bernhard-Nocht-Institut für Tropenmedizin, Hamburg, Germany

author

Andreas Niebuhr

• Abteilung für Virologie, Bernhard-Nocht-Institut für Tropenmedizin, Hamburg, Germany

author

Annemarie Haag

• Abteilung für Virologie, Bernhard-Nocht-Institut für Tropenmedizin, Hamburg, Germany

author

Herbert Schmitz

• Abteilung für Virologie, Bernhard-Nocht-Institut für Tropenmedizin, Hamburg, Germany

author

Julian Schulze zur Wiesch

• Klinik und Poliklinik Innere Medizin, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany

author

Joonho Choe

• Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejeon, Korea

author

Maria A Siwecka

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

author

Tadeusz Kulikowski

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

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