Chemical characterization of effective and ineffective strains of Rhizobium leguminosarum bv. viciae

• Authors: Stanislav F. Izmailov , Genrietta Ya. Zhiznevskaya , Larisa V. Kosenko , Galina N. Troitskaya , Nataly N. Kudryavtseva , Ludmila I. Borodenko , Pavel N. Dubrovo , Ryszard Russa , Hubert Pietras , Zbigniew Lorkiewicz
• Languages of publication: EN

Abstracts

EN

Chemical composition of lipopolysaccharide (LPS) isolated from an effective (97) and ineffective (87) strains of R. l. viciae has been determined. LPS preparations from the two strains contained: glucose, galactose, mannose, fucose, arabinose, heptose, glucosamine, galactosamine, quinovosamine, and 3-N-methyl-3,6-dideoxyhexose, as well as glucuronic, galacturonic and 3-deoxyoctulosonic acid. The following fatty acids were identified: 3-OH 14:0, 3-OH 15:0, 3-OH 16:0, 3-OH 18:0 and 27-OH 28:0. The ratio of 3-OH 14:0 to other major fatty acids  in LPS 87 was higher that in LPS 97. SDS/PAGE profiles of LPS indicated that, in lipopolysaccharides, relative content of S form LPS I to that of lower molecular mass (LPS II) was much higher in the effective strain 97 than in 87. All types of polysaccharides exo-, capsular-, lipo, (EPS, CPS, LPS, respectively) examined possessed the ability to bind faba bean lectin. The degree of affinity of the host lectin to LPS 87 was half that to LPS 97. Fatty acids (FA) composition from bacteroids and peribacteroid membrane (PBM) was determined. Palmitic, stearic and hexadecenoic acids were common components found in both strains. There was a high content of unsaturated fatty acids in bacteroids as well as in PBM lipids. The unsaturation index in the PBM formed by strain 87 was lower than in the case of strain 97. Higher ratio of 16:0 to 18:1 fatty acids was characteristic for PMB of the ineffective strain.

Keywords

EN

lipopolysaccharides; fatty acids; Rhizobium leguminosarum bv. viciae

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 1999
• Volume: 46
• Issue: 4
• Pages: 1001-1009

Dates

published

1999

received

1999-09-03

Contributors

author

Stanislav F. Izmailov

• Timiriazev Institute of Plant Physiology, Russian Academy of Sciences, 35 Botanicheskaya, 127276 Moscow, Russia

author

Genrietta Ya. Zhiznevskaya

• Timiriazev Institute of Plant Physiology, Russian Academy of Sciences, 35 Botanicheskaya, 127276 Moscow, Russia

author

Larisa V. Kosenko

• Institute of Microbiology and Virology, Ukrainian Academy of Sciences, 154 Zabolotny, Kyiv-143, 252143 , Ukraine

author

Galina N. Troitskaya

• Timiriazev Institute of Plant Physiology, Russian Academy of Sciences, 35 Botanicheskaya, 127276 Moscow, Russia

author

Nataly N. Kudryavtseva

• Timiriazev Institute of Plant Physiology, Russian Academy of Sciences, 35 Botanicheskaya, 127276 Moscow, Russia

author

Ludmila I. Borodenko

• Timiriazev Institute of Plant Physiology, Russian Academy of Sciences, 35 Botanicheskaya, 127276 Moscow, Russia

author

Pavel N. Dubrovo

• Timiriazev Institute of Plant Physiology, Russian Academy of Sciences, 35 Botanicheskaya, 127276 Moscow, Russia

author

Ryszard Russa

• Department of General Microbiology, Marie Curie-Skłodowska University, 19 Akademicka, 20-033 Lublin, Poland

author

Hubert Pietras

• Department of General Microbiology, Marie Curie-Skłodowska University, 19 Akademicka, 20-033 Lublin, Poland

author

Zbigniew Lorkiewicz

• Department of General Microbiology, Marie Curie-Skłodowska University, 19 Akademicka, 20-033 Lublin, Poland