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Number of results
2012 | 33 | 4 | 651-665

Article title

Kinetic Modelling of Lovastatin Biosynthesis by Aspergillus Terreus Cultivated on Lactose and Glycerol as Carbon Sources

Content

Title variants

Languages of publication

EN

Abstracts

EN
A kinetic model to describe lovastatin biosynthesis by Aspergillus terreus ATCC 20542 in a batch culture with the simultaneous use of lactose and glycerol as carbon sources was developed. In order to do this the kinetics of the process was first studied. Then, the model consisting of five ordinary differential equations to balance lactose, glycerol, organic nitrogen, lovastatin and biomass was proposed. A set of batch experiments with a varying lactose to glycerol ratio was used to finally establish the form of this model and find its parameters. The parameters were either directly determined from the experimental data (maximum biomass specific growth rate, yield coefficients) or identified with the use of the optimisation software. In the next step the model was verified with the use of the independent sets of data obtained from the bioreactor cultivations. In the end the parameters of the model were thoroughly discussed with regard to their biological sense. The fit of the model to the experimental data proved to be satisfactory and gave a new insight to develop various strategies of cultivation of A. terreus with the use of two substrates.

Publisher

Year

Volume

33

Issue

4

Pages

651-665

Physical description

Dates

published
1 - 12 - 2012
online
28 - 12 - 2012

Contributors

author
  • Łódź University of Technology, Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Wolczanska 213, Lodz, Poland
  • Łódź University of Technology, Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Wolczanska 213, Lodz, Poland

References

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  • Bizukojć M., Ledakowicz S., 2007. A macrokinetic modelling of the biosynthesis of lovastatin by Aspergillus terreus. J. Biotechnol. 130, 422-435. DOI: 10.1016/j.jbiotec.2007.05.007.[WoS][Crossref]
  • Bizukojć M., Pawłowska B., Ledakowicz S., 2007. Supplementation of the cultivation media with B-group vitamins enhances lovastatin biosynthesis by Aspergillus terreus. J. Biotechnol. 127, 258-268. DOI: 10.1016/j.jbiotec.2006.06.017.[Crossref][WoS]
  • Bizukojć M., Pecyna M., 2011. Lovastatin and (+)-geodin formation by Aspergillus terreus ATCC 20542 in a batch culture with the simultaneous use of lactose and glycerol as carbon sources. Eng. Life Sci. 11, 272-282. DOI: 10.1002/elsc.201000179.
  • Casas López J.L., Sánchez Pérez J.A., Fernández Sevilla J.M., Acién Fernández F.G., Molina Grima E., Chisti Y., 2003. Production of lovastatin by Aspergillus terreus: effects of C:N ratio and the principal nutrients on growth and metabolite production. Enzyme Microb. Technol., 33, 270-277. DOI: 10.1016/S0141-0229(03)00130-3.[Crossref]
  • Casas López J.L., Sánchez Pérez J.A., Fernández Sevilla J.M., Rodríguez Porcel E.M., Chisti Y., 2005. Pellet morphology, culture rheology and lovastatin production in cultures of Aspergillus terreus. J. Biotechnol., 116, 61-77. DOI: 10.1016/j.jbiotec.2004.10.005.[Crossref]
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  • Pawlak M., Bizukojć M., Ledakowicz S., 2012. Impact of bioreactor scale on lovastatin biosynthesis by Aspergillus terreus ATCC 20542 in a batch culture. Chem. Proc. Eng., 33, 71-84. DOI: 10.2478/v10176-012- 0007-0.
  • Pecyna M., Bizukojć M., 2011. Lovastatin biosynthesis by Aspergillus terreus with the simultaneous use of lactose and glycerol in a discontinuous fed-batch culture J. Biotechnol. 151, 77-86. DOI: 10.1016/j.jbiotec.2010.10.079.[Crossref][WoS]
  • Rodríguez Porcel E.M., Casas López J.L., Sánchez Pérez J.A., Fernández Sevilla J.M., García Sánchez J.L., Chisti Y., 2006. Aspergillus terreus broth rheology, oxygen transfer, and lovastatin production in a gas-agitated slurry reactor. Ind. Eng. Chem. Res. 45, 4837-4843. DOI: 10.1021/ie0600801.[Crossref]
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Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.-psjd-doi-10_2478_v10176-012-0055-5
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