Substrate Inhibition in Lipase-Catalysed Transesterification of Mandelic acid with Vinyl Acetate

• Authors: Katarzyna Dąbkowska , Maciej Pilarek , Krzysztof W. Szewczyk
• Languages of publication: EN

Abstracts

EN

Kinetic resolution of (R)- and (S)-mandelic acid by its transesterification with vinyl acetate catalysed by Burholderia cepacia lipase has been studied. The influence of the initial substrate concentration on the kinetics of process has been investigated. A modified ping-pong bi-bi model of enzymatic transesterification of (S)-mandelic acid including substrate inhibition has been developed. The values of kinetic parameters of the model have been estimated. We have shown that the inhibition effect revealed over a certain threshold limit value of the initial concentration of substrate.

Keywords

EN

kinetic resolution; enantiomers; kinetic model; lipase; substrate inhibition

• Publisher: De Gruyter Open
• Journal: Chemical and Process Engineering
• Year: 2012
• Volume: 33
• Issue: 4
• Pages: 539-546

Dates

published

1 - 12 - 2012

online

28 - 12 - 2012

Contributors

author

Katarzyna Dąbkowska

• Warsaw University of Technology, Faculty of Chemical and Process Engineering, Department of Biotechnology and Bioprocess Engineering, Waryńskiego 1, 00-645 Warszawa, Poland

author

Maciej Pilarek

• Warsaw University of Technology, Faculty of Chemical and Process Engineering, Department of Biotechnology and Bioprocess Engineering, Waryńskiego 1, 00-645 Warszawa, Poland

author

Krzysztof W. Szewczyk

• Warsaw University of Technology, Faculty of Chemical and Process Engineering, Department of Biotechnology and Bioprocess Engineering, Waryńskiego 1, 00-645 Warszawa, Poland

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Identifiers

DOI

10.2478/v10176-012-0044-8