Determination of phase diagrams and thermoseparation behaviour of aqueous two-phase systems composed of ethylene oxide–propylene oxide random copolymer and potassium phosphate

• Authors: Radosław Dembczyński , Wojciech Białas , Tomasz Jankowski
• Languages of publication: EN

Abstracts

EN

The application of aqueous two-phase systems (ATPS) is a cost-effective and simple method of protein separation (including enzymes) from complex systems. The first stage of designing the protein purification process in an ATPS involves the identification of the conditions for the formation of a given extraction system. For this purpose, the conditions for the formation of ATPSs in a thermoseparating EO50PO50 polymer/potassium phosphates system have been studied. Factors determining the ATPS formation comprised: separation temperature (4ºC or 20ºC), phosphate solution pH (6, 7.5 or 9) as well as the concentration of NaCl introduced into the systems (0.085 M, 0.475 M and 0.85 M). ATPS without NaCl were prepared as well. The conditions for the formation of the primary EO50PO50/potassium phosphate ATPS were determined with their phase diagrams. It was observed that with an increase of phosphate pH and NaCl concentration in the system, there was a decrease of the EO50PO50 and phosphate concentrations necessary to form a primary ATPS. After the primary two-phase separation, the top phase (rich in the EO50PO50 polymer) was partitioned from the bottom phase (rich in phosphates). Next, by means of polymer phase thermoseparation, a secondary two-phase system was formed. In the secondary EO50PO50/phosphate ATPS, the bottom phase was formed by the concentrated EO50PO50 polymer (30-80% concentration), while the top phase by a solution composed mainly of water, containing phosphate ions and remains of EO50PO50 polymer (3-7%).

Keywords

EN

aqueous two-phase systems; phase diagrams; thermoseparation; partition coefficient; ethylene oxide-propylene oxide copolymers

• Publisher: De Gruyter Open
• Journal: Chemical and Process Engineering
• Year: 2012
• Volume: 33
• Issue: 3
• Pages: 411-429

Dates

published

1 - 10 - 2012

received

accepted

online

31 - 10 - 2012

Contributors

author

Radosław Dembczyński

• Poznan University of Life Sciences, Faculty of Food Science and Nutrition, Department of Biotechnology and Food Microbiology, Wojska Polskiego 48, 60 – 627 Poznań, Poland

author

Wojciech Białas

• Poznan University of Life Sciences, Faculty of Food Science and Nutrition, Department of Biotechnology and Food Microbiology, Wojska Polskiego 48, 60 – 627 Poznań, Poland

author

Tomasz Jankowski

• Poznan University of Life Sciences, Faculty of Food Science and Nutrition, Department of Biotechnology and Food Microbiology, Wojska Polskiego 48, 60 – 627 Poznań, Poland

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Identifiers

DOI

10.2478/v10176-012-0036-8