Do G894T Polymorphisms of Endothelial Nitric Oxide Synthase 3 (NOS3) Influence Endurance Phenotypes?

• Authors: Paweł Cięszczyk , Marek Sawczuk , Agnieszka Maciejewska , Nijole Jascaniene , Jerzy Eider
• Languages of publication: EN

Abstracts

EN

Endothelial nitric oxide (NO) synthase gene (NOS3) is taken into account as one of the main regulators of blood pressure and basal vascular dilation - two main factors found to be limiting for endurance performance.We compared genotypic and allelic frequencies of the NOS3 G894T polymorphism in two groups of men of the same Caucasian descent: elite endurance athletes (rowers; n=63) and sedentary controls (n=160).We have not found any statistical difference in G894T genotype and allele frequencies in endurance orientated athletes compared to sedentary controls. The difference in G allele frequency between the rowers and controls did not reach statistical significance (73.5% vs. 67.2%, P = 0.307), similar to genotype distribution amongst the rowers (58.7% GG; 39.4% GT; 6.4% TT) compared to controls (43.7% GG; 46.9% GT; 9.4% TT) (P=0.129).In summary, our results are in contradiction to the hypothesis that NOS3 G894T polymorphism is associated with the physical performance status in rowing. Of course, our findings do not mean that other polymorphisms in NOS3 gene do not have any beneficial effect on performance parameters, but to confirm that hypothesis, we need further studies.

Keywords

EN

NOS3; genotype; rowing; endurance performance

• Publisher: De Gruyter Open
• Journal: Journal of Human Kinetics
• Year: 2010
• Volume: 24
• Pages: 73-80

Dates

published

1 - 1 - 2010

online

21 - 6 - 2010

Contributors

author

Paweł Cięszczyk

• Institute of Physical Culture, University of Szczecin, Poland

author

Marek Sawczuk

• Department of Genetics, University of Szczecin, Poland

author

Agnieszka Maciejewska

• Department of Genetics, University of Szczecin, Poland

author

Nijole Jascaniene

• Vilnius Pedagogical University, Studentu St. 39, LT-08106 Vilnius, Lithuania

author

Jerzy Eider

• Institute of Physical Culture, University of Szczecin, Poland

References

• Ahmetov II, Rogozkin VA, Genes, Athlete Status and Training - An Overview in Genetics and Sports (ed: Callins M). Med Sport Sci. Basel, Karger, 2009, vol 54, pp 43-71.
• Borggrefe M, Wolpert C, Antzelevitch C, Veltmann C, Giustetto C, Gaita F, Schimpf R. Short QT syndrome. Genotype-phenotype correlations. J Electrocardiol 2005: 38: 75-80.
• Bray MS, Hagberg JM, Perusse L, Rankinen T, Roth SM, Wolfarth B, Bouchard C (2009) The human gene map for performance and health-related fitness phenotypes: the 2006-2007 update. Med Sci Sports Exerc 41:35-73[Crossref][PubMed]
• Cieszczyk P., Krupceki K, Maciejewska A., Sawczuk M.:. The Angiotensin Converting Enzyme Gene I/D Polymorphism in Polish Rowers. International Journal of Sports Medicine. 2009; 30: 624-627
• Gomez-Gallego F., Ruiz JR., Buxens A., Artieda M., Arteta D., Santiago Ć., Rodriguez-Romo G., Lao J. I., Lucia A., The 2786 T/C polymorphism of the NOS3 gene is associated with elite performance in power sports. Eur J Appl Physiol 2009 107:565-569[WoS]
• Harrison DG., Cai H. Endothelial control of vasomotion and nitric oxide peoduction. Cardiol Clin 2003; 21: 289-302
• Hand BD, McCole SD, Brown MD, Park JJ, Ferrell RE, Huberty A, Douglass LW, Hagberg JM. NOS3 gene polymorphisms and exercise hemodynamics in postmenopausal women. Int J Sports Med 2006: 27: 951-958.[PubMed]
• Higashi Y, Sasaki S, Sasaki N, Nakagawa K, Ueda T, Yoshimizu A, Kurisu S, Matsuura H, Kajiyama G, Oshima T. Daily aerobic exercise improves reactive hyperemia in patients with essential hypertension. Hypertension 1999: 33: 591-597.[PubMed]
• Jones AM, Wilkerson DP, Wilmshurst S, Campbell IT. Influence of L-NAME on pulmonary O2 uptake kinetics during heavy-intensity cycle exercise. J Appl Physiol 2004: 96: 1033-1038.[PubMed]
• Kimura T., Yokoyama T., Matsumura Y., Yoshiike N., Date C., Muramatsu M., Tanaka H., NOS3 Genotype-Dependent Correlation Between Blood Pressure and Physical Activity, Hypertension 2003;41;355-360;[WoS]
• Long JH, Lira VA, Soltow QA, Betters JL, Sellman JE, Criswell DS. Arginine supplementation induces myoblast fusion via augmentation of nitric oxide production. J Muscle Res Cell Motil 2006, 27:577-584
• Massion PB, Feron O, Dessy C, Balligand JL. Nitric oxide and cardiac function: ten years after, and continuing. Circ Res 2003: 93: 388-398.
• McConell GK, Kingwell BA () Does nitric oxide regulate skeletal muscle glucose uptake during exercise? Exerc Sport Sci Rev 2006, 34:36-41[WoS]
• Otani H (2009) The role of nitric oxide in myocardial repair and remodeling. Antioxid Redox Signal [Epub ahead of print]
• Quyyumi AA, Dakak N, Andrews NP, Gilligan DM, Panza JA, Cannon RO 3rd (1995) Contribution of nitric oxide to metabolic coronary vasodilation in the human heart. Circulation 92:320-326
• Rankinen T, Rice T, Perusse L, Chagnon YC, Gagnon J, Leon AS, Skinner JS, Wilmore JH, Rao DC, Bouchard C. NOS3 Glu298Asp genotype and blood pressure response to endurance training: the HERITAGE family study. Hypertension. 2000; 36:885-889.
• Robinson LJ, Weremowicz S, Morton CC, Michel T () Isolation and chromosomal localization of the human endothelial nitric oxide synthase (NOS3) gene. Genomics 1994, 19:350-357
• Shen, W., Zhang, X., Zhao, G., Wolin, M. S., Sessa, W. and Hintze, T. H. () Nitric oxide production and NO synthase gene expression contribute to vascular regulation during exercise. Med. Sci. Sports Exerc., 1995, 27, 1125-1134.
• Spina RJ, Turner MJ, Ehsani AA. Exercise training enhances cardiac function in response to an afterload stress in older men. Am J Physiol 1997, 272: H995-1000.
• Tsukada T, Yokoyama K, Arai T, Takemoto F, Hara S, Yamada A, Kawaguchi Y, Hosoya T, Igari J. Evidence of association of the ecNOS gene polymorphism with plasma NO metabolite levels in humans. Biochem Biophys Res Commun. 1998, 245:190-193.
• Wang XL, Sim AS, Wang MX, Murrell GA, Trudinger B, Wang J () Genotype dependent and cigarette specific effects on endothelial nitric oxide synthase gene expression and enzyme activity. FEBS Lett 2000, 471:45-50
• Wilkerson DP, Campbell IT, Jones AM () Influence of nitric oxide synthase inhibition on pulmonary O2 uptake kinetics during supra-maximal exercise in humans. J Physiol 2004, 561:623-635
• Williams AG, Dhamrait SS, Wootton PT, Day SH, Hawe E, Payne JR, Myerson SG, World M, Budgett R, Humphries SE, Montgomery HE. Bradykinin receptor gene variant and human physical performance. J Appl Physiol 2004, 96: 938-942.
• Wolfarth B., Rankinen T., Muhlbauer S., Ducke M., Rauramaa R., Boulay M. R., Perusse L., Bouchard C. Endothelial nitric oxide synthase gene polymorphism and elite endurance athlete status: the Genathlete study. Scand J Med Sci Sports 2008, 18:485-490[WoS]
• Wolfarth B, Rivera MA, Oppert JM, Boulay MR, Dionne FT, Chagnon M, Gagnon J, Chagnon Y, Perusse L, Bouchard C. A polymorphism in the alpha2a-adrenoceptor gene and endurance athlete status. Med Sci Sports Exerc 2000, 32: 1709-1712.

Identifiers

DOI

10.2478/v10078-010-0022-5