Inhibition of Selected Enzymes with Specific Inhibitors in the Tissue Homogenates from Patients with Colorectal Cancer

• Authors: Andrzej Hap , Wojciech Kielan , Maciej Siewiński , Robert Tarnawa , Anil Agrawal , Julia Rudno-Rudzińska , Wojciech Hap , Zygmunt Grzebieniak
• Languages of publication: EN

Abstracts

EN

Large intestine malignancy is the second most common malignancy and second leading cause of cancer mortality in Poland. This is related to late detection of these lesions, e.g. due to lack of effective screening tests. Lesions found by a surgeon are clinically advanced, making the treatment often ineffective and sometimes even completely impossible. Discovery of a substance that would be able to stop key processes for the development of malignancy could change such situation. Activity of certain enzymes was found to increase in malignant cells and invasion of malignancy could be triggered by inadequate amount of endogenous inhibitors of these enzymes in the surrounding healthy tissues. Inhibitors identical with that produced in human cells were found in egg whites.The aim of the study was to determine ability of cystatin isolated from egg whites to inhibit activity of cathepsin B and L.Material and methods. Immunohistochemistry and histology of tissue specimen collected from malignant lesions resected from 60 patients diagnosed with large intestine adenocarcinoma, who underwent surgical treatment in 2nd Department of General and Oncological Surgery, Medical University of Wrocław between 2007 and 2009.Results. Differences were fund between health tissues, margins and center of the malignant lesions with regard to amount and distribution of stained cathepsin B - cystatin complexes. The above mentioned inhibitors were able to inhibit 90% of primary activity of cathepsin B and L in malignant tissues.Conclusions. Cystatins obtained from egg whites could be used as substances supporting anti-cancer therapy in the future.

Keywords

EN

malignancy; large intestine; cystatins; cathepsins; inhibitor therapy

• Publisher: Index Copernicus International
• Journal: Polish Journal of Surgery
• Year: 2010
• Volume: 82
• Issue: 9
• Pages: 504-509

Dates

published

1 - 9 - 2010

online

22 - 10 - 2010

Contributors

author

Andrzej Hap

• Department of Surgery, Regional Hospital in Legnica

author

Wojciech Kielan

• 2 Department of General and Oncological Surgery, Medical University in Wrocław

author

Maciej Siewiński

• Department of Basic Science Faculty, Public Health Medical University in Wrocław

author

Robert Tarnawa

• 2 Department of General and Oncological Surgery, Medical University in Wrocław

author

Anil Agrawal

• 2 Department of General and Oncological Surgery, Medical University in Wrocław

author

Julia Rudno-Rudzińska

• 2 Department of General and Oncological Surgery, Medical University in Wrocław

author

Wojciech Hap

• Student Research Group - 2 Department of General and Oncological Surgery, Medical University in Wrocław

author

Zygmunt Grzebieniak

• 2 Department of General and Oncological Surgery, Medical University in Wrocław

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Identifiers

DOI

10.2478/v10035-010-0073-2