The - 801 G/A Polymorphism of CXCL12 Promoter Gene as Unfavorable Genetic Prognosis factor involved in Colorectal Cancer

• Authors: Ewa Langner , Karolina Przybyłowska , Andrzej Sygut , Michał Mik , Łukasz Dziki , Mirosław Dziekiewicz , Ireneusz Majsterek , Adam Dziki
• Languages of publication: EN

Abstracts

EN

CCXL12 also called stromal derived factor-1 (SDF-1), a protein related to angiogenesis and inflammation, has been correlated with the progression of a number of malignancies. Single nucleotide - 801G/A polymorphism of CXCL12 gene has been described and is regarded as a target for cis-acting factor that has the ability to up-regulate CXCL12 expression.The aim of the study. Based on the suggested role of CXCL12 in the pathogenesis of cancer we examined the association of the gene variant CXCL12-A with colorectal cancer.Material and methods. We genotyped - 801G/A polymorphism of CXCL12 gene in 164 colorectal patients and 184 age-matched healthy subjects. Genotyping was done with PCR-RFLP.Results. There were no significant differences in the frequencies of SDF1-3'A allele, between patients and controls. The frequency of CXCL12 G/A and G/A plus A/A genotype was significantly higher in a group of patients with lymph node metastasis compared with those without metastasis.Conclusions. The CXCL12 gene G/A polymorphism was not related to colorectal cancer risk but is associated with the induction of lymph-node metastasis of colorectal cancer disease in Polish.

Keywords

EN

colorectal cancer; - 801 G/A polymorphism; CXCL 12 gene

• Publisher: Index Copernicus International
• Journal: Polish Journal of Surgery
• Year: 2010
• Volume: 82
• Issue: 8
• Pages: 459-463

Dates

published

1 - 8 - 2010

online

22 - 9 - 2010

Contributors

author

Ewa Langner

• Department of General and Colorectal Surgery, Medical University, Łódź

author

Karolina Przybyłowska

• Department of Clinical Chemistry and Biochemistry, Medical University, Łódź

author

Andrzej Sygut

• Department of General and Colorectal Surgery, Medical University, Łódź

author

Michał Mik

• Department of General, Oncological and Vascular Surgery, Military Institute of Medicine, Warsaw

author

Łukasz Dziki

• Department of General and Colorectal Surgery, Medical University, Łódź

author

Mirosław Dziekiewicz

• Department of Clinical Chemistry and Biochemistry, Medical University, Łódź

author

Ireneusz Majsterek

• Department of General and Colorectal Surgery, Medical University, Łódź

author

Adam Dziki

• Department of General and Colorectal Surgery, Medical University, Łódź

References

• Burger M, Glodek A, Hartmann T et al.: Functional expression of CXCR4 (CD184) on small-cell lung cancer cells mediates migration, integrin activation, and adhesion to stromal cells. Oncogene 2003; 22: 8093-01.
• Klein RS, Rubin JB, Gibson HD et al.: SDF-1_induces chemotaxis and enhances Sonic hedgehog-induced proliferation of cerebellar granule cells. Development. 2001; 128: 1971-81.
• Raman D, Baugher PJ, Thu YM et al.: Role of chemokines in tumor growth. Cancer Lett 2007; 256(2): 137-65.
• Zeelenberg IS, Ruuls-Van Stalle L, Roos E: The chemokine receptor CXCR4 is required for outgrowth of colon carcinoma micrometastases. Cancer Res 2003; 63: 3833-39.
• Burger JA, Kipps TJ: a key receptor in the crosstalk between tumor cells and their microenvironment. Blood 2006; 107(5): 1761-67.[Crossref][PubMed]
• Fernandis AZ, Prasad A, Band H et al.: Regulation of CXCR4-mediated chemotaxis and chemoinvasion of breast cancer cells. Oncogene 2004; 23: 157-67.[Crossref]
• Taichman RS, Cooper C, Keller ET et al.: Use of the stromal cellderived factor-1/CXCR4 pathway in prostate cancer metastasis to bone. Cancer Res 2002; 62: 1832-37.
• Wendt MK, Johanesen PA, Kang-Decker N et al.: Silencing of epithelial CXCL12 expression by DNA hypermethylation promotes colonic carcinoma metastasis. Oncogene 2006, 25: 4986-97.[Crossref]
• Yoshitake N, Fukui H, Yamagishi H et al.: Expression of SDF-1 alpha and nuclear CXCR4predicts lymph node metastasis in colorectal cancer. Br J Cancer 2008; 98(10): 1682-89.[Crossref]
• Kim J, Mori T, Chen SL et al.: Chemokine receptor CXCR4 expression in patients with melanoma and colorectal cancer liver metastases and the association with disease outcome. Ann Surg 2006; 244(1): 113-20.
• Kollmar O, Rupertus K, Scheuer C et al.: Stromal cell-derived factor-1 promotes cell migration and tumor growth of colorectal metastasis. Neoplasia 2007; 9(10): 862-70.[WoS]
• Winkler C, Modi W, Smith MW et al.: Geneticr estriction of AIDS pathogenesis by an chemokine gene variant. ALIVE Study, Hemophilia Growth and Development Study (HGDS), Multicenter AIDS Cohort Study (MACS), Multicenter Hemophilia Cohort Study (MHCS), San Francisco City Cohort (SFCC). Science 1998; 279: 389-93.
• Watanabe MA, de Oliveia Cavassin GG, Orellana MD et al.: SDF-1 gene polymorphisms and syncytia induction in Brazilian HIV-1 infected individuals. Microb Pathog 2003; 35: 31-34.[Crossref]
• Hirata H, Hinoda Y, Kikuno N et al.: CXCL12 G801A polymorphism is a risk factor for sporadic prostate cancer susceptibility. Clin Cancer Res 2007; 13(17): 5056-62.
• Razmkhah M, Talei AR, Doroudchi M et al.: Stromal cell-derived factor-1 (SDF-1) alleles and susceptibility to breast carcinoma. Cancer Lett 2005; 225: 261-66.
• Razmkhah M, Doroudchi M, Ghayumi SM et al.: Stromal cell-derived factor-1 (SDF-1) gene and susceptibility of Iranian patients with lung cancer. Lung Cancer 2005; 49: 311-15.[Crossref][PubMed]
• Teng YH, Liu TH, Tseng HC et al.: Contribution of genetic polymorphisms of stromal cell-derived factor-1 and its receptor, CXCR4, to the susceptibility and clinicopathologic development of oral cancer. Head Neck 2009; 31(10): 1282-88.[WoS]
• Chang CC, Chen SC, Hsieh YH et al.: Stromal cell-derived factor-1 but not its receptor, CXCR4, gene variants increase susceptibility and pathological development of hepatocellular carcinoma. Clin Chem Lab Med 2009; 47(4): 412-18.[WoS]
• Dimberg J, Hugander A, Löfgren S et al.: Polymorphism and circulating levels of the chemokine CXCL12 in colorectal cancer patients. Int J Mol Med 2007; 19(1): 11-15.
• Hidalgo-Pascual M, Galan JJ, Chaves-Conde M et al.: Analysis of CXCL12 3'UTR G>A polymorphism in colorectal cancer. Oncol Rep 2007; 18(6): 1583-87.
• Vairaktaris E, Vylliotis A, Spyridonodou S et al.: A DNA polymorphism of stromal-derived factor-1 is associated with advanced stages of oral cancer. Anticancer Res 2008; 28(1A): 271-75.

Identifiers

DOI

10.2478/v10035-010-0066-1