The influence of low temperatures on dynamic mechanical properties of animal bone

• Authors: Marcin Mardas , Leszek Kubisz , Slawomir Mielcarek , Piotr Biskupski
• Languages of publication: EN

Abstracts

EN

Different preservation methods are currently used in bone banks, even though their effects on allograft quality are not fully understood. Freezing is one of the most popular methods of preservation in tissue banking. Yet, there is not a lot of data on dynamic mechanical properties of frozen bone. Material used in this study was femoral bones from adult bovine that were machine cut and frozen to the temperature 140°C. Both elastic modulus and loss modulus were measured at 1, 3, 5, 10, and 20 Hz in the temperature range of 30-200°C. Differences between frozen and control samples were observed. The frequency increase always led to the increase in elastic modulus values and decrease in loss modulus values. Freezing reduced the elastic modulus values of about 25% and the loss modulus values of about 45% when measured at 20°C.

Keywords

EN

dynamic mechanical analysis; bones; freezing

• Publisher: Versita
• Journal: Polish Journal of Medical Physics And Engineering
• Year: 2009
• Volume: 15
• Issue: 3
• Pages: 153-159

Dates

published

1 - 1 - 2009

online

22 - 9 - 2010

Contributors

author

Marcin Mardas

• Department of Biophysics, University of Medical Sciences, Poznan, Poland

author

Leszek Kubisz

• Department of Biophysics, University of Medical Sciences, Poznan, Poland

author

Slawomir Mielcarek

• Institute of Physics, Adam Mickiewicz University, Poznan, Poland

author

Piotr Biskupski

• Institute of Physics, Adam Mickiewicz University, Poznan, Poland

References

• Akkus O, Belaney RM, Das P. Free radical scavenging alleviates the biomechanical impairment of gamma radiation sterilized bone tissue. J Orthop Res. 2005; 23(4): 838-45.[Crossref][PubMed]
• Andrade MG, Sa CN, Marchionni AM, de Bittencourt TC, Sadigursky M. Effects of freezing on bone histological morphology. Cell Tissue Bank. 2008. (Epub)[WoS]
• Balsly CR, Cotter AT, Williams LA, Gaskins BD, Moore MA, Wolfinbarger L Jr. Effect of low dose and moderate dose gamma irradiation on the mechanical properties of bone and soft tissue allografts. Cell Tissue Bank. 2008. (Epub)[PubMed][WoS]
• Butler AM, Morgan DA, Verheul R, Walsh WR. Mechanical properties of gamma irradiated morselized bone during compaction. Biomaterials. 2005; 26(30): 6009-13.[PubMed][Crossref]
• Cornu O, Bavadekar A, Godts B, Van Tomme J, Delloye C, Banse X. Impaction bone grafting with freeze-dried irradiated bone. Part II. Changes in stiffness and compactness of morselized grafts: experiments in cadavers. Acta Orthop Scand. 2003; 74(5): 553-8.[PubMed][Crossref]
• Frankel V. The femoral neck. Uppsala, Almguist and Wiksell, 1960.
• Grieb TA, Forng RY, Stafford RE, Lin J, Almeida J, Bogdansky S, Ronholdt C, Drohan WN, Burgess WH. Effective use of optimized, high-dose (50 kGy) gamma irradiation for pathogen inactivation of human bone allografts. Biomaterials. 2005; 26(14): 2033-42.[PubMed][Crossref]
• Hamer AJ, Stockley I, Elson RA. Changes in allograft bone irradiated at different temperatures. J Bone Joint Surg Br. 1999; 81(2): 342-4.[Crossref][PubMed]
• Kang JS, Kim NH. The biomechanical properties of deep freezing and freeze drying bones and their biomechanical changes after in-vivo allograft. Yonsei Med J. 1995; 36(4): 332-5.[PubMed]
• Komender A. Influence of preservation on some mechanical properties of human haversian bone. Mater Med Pol. 1976; 8: 13.[PubMed]
• Kubisz L, Mielcarek S, Biskupski P, Mardas M. Dynamic mechanical properties of gamma sterilized Animals compact bone. Pol J Environ Stud. 2006; 15(4A): 72-74.
• Malinin T, Temple HT. Comparison of frozen and freeze-dried particulate bone allografts. Cryobiology. 2007; 55(2): 167-70.[WoS][PubMed][Crossref]
• Menard KP. Dynamic mechanical analysis: a practical introduction. CRC Press. 1999.
• Moreno J, Forriol F. Effects of preservation on the mechanical strength and chemical composition of cortical bone: an experimental study in sheep femora. Biomaterials. 2002; 23(12): 2615-9.[Crossref]
• Nguyen H, Morgan DA, Forwood MR. Sterilization of allograft bone: is 25 kGy the gold standard for gamma irradiation? Cell Tissue Bank. 2007; 8(2): 81-91.[Crossref][PubMed]
• Nguyen H, Morgan DA, Forwood MR. Sterilization of allograft bone: effects of gamma irradiation on allograft biology and biomechanics. Cell Tissue Bank. 2007; 8(2): 93-105.[PubMed][Crossref]
• Pelker RR, Friedlaender GE, Markham TC. Biomechanical properties of bone allografts. Clin Orthop. 1983; 174: 54-57.
• Pelker R, Markham T, Friedlaender G, Moen C, Panjabi M. The effect of preservation on allograft strength. Trans Orthop Res Soc. 1982; 7: 283.
• Sedin E. A rheologic model for cortical bone. Acta Orthop Scand. 1965; 36 Suppl: 83.
• Yamashita J, Furman BR, Rawls R, Wang X, Agrawal CM. The use of dynamic mechanical analysis to asses the viscoelastic properties of human cortical bone. J Biomed Mater Res. 2001; 58: 47-53.[Crossref]

Identifiers

DOI

10.2478/v10013-009-0015-7