Scutellarein ameliorates tongue cancer cells via mitochondria

• Authors: Guangping Jing , Jinhua Zheng , Xueyong Wang , Haixia Li , Xiaohui Jiao
• Languages of publication: EN

Abstracts

EN

To investigate the mechanism in Scutellarein-induced apoptosis of SAS human tongue cancer cells, inhibitory effects of Scutellarein on SAS cells were detected by MTT assay. Cell apoptosis was analyzed by flow cytometry. Ultrastructural changes of SAS cells were observed by transmission electron microscopy (TEM). Mitochondrial transmembrane potential (ΔΨm) were analyzed by JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide]. Western blotting was used to examine the expression level of Bcl-2, Bax and caspase-3 in SAS cells treated with Scutellarein. Scutellarein inhibited the proliferation of SAS cells in a time and dose-dependent manner and increased the percent of apoptotic cells. The mitochondrial cristae were swollen and had vacuolar degeneration. ΔΨm decreased when the concentration of scutellarein increased. Scutellarein effectively up-regulated the expression of mitochondrial Bax and caspase-3 and down-regulated the expression of Bcl-2. Scutellarein induces apoptosis of SAS human tongue cancer cells via activating mitochondrial signaling pathway.

Keywords

EN

Scutellarein; Mitochondria; Apoptosis; SAS Tongue cancer cell

• Publisher: De Gruyter Open
• Journal: Open Medicine
• Year: 2014
• Volume: 9
• Issue: 2
• Pages: 193-199

Dates

published

1 - 4 - 2014

online

13 - 2 - 2014

Contributors

author

Guangping Jing

• Department of Oral Anatomy & Physiology, Stomatological College, Harbin Medical University, Harbin, 150001, China

author

Jinhua Zheng

• Department of Anatomy, Basic Medical Science College, Harbin Medical University, Harbin, 150001, China

author

Xueyong Wang

• Department of Anatomy, Basic Medical Science College, Harbin Medical University, Harbin, 150001, China

author

Haixia Li

• Department of Anatomy, Basic Medical Science College, Harbin Medical University, Harbin, 150001, China

author

Xiaohui Jiao

• Department of Oral & Maxillofacial Surgery, Stomatological College, Harbin Medical University, Harbin, 150001, China

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Identifiers

DOI

10.2478/s11536-013-0269-z