Testing endodontic sealers on human umbilical vein endothelial cells

• Authors: Marioara Moldovan , Adriana Filip , Cristina Prejmerean , Doina Prodan , Camelia Alb , Aniela Pop , Baldea Ioana
• Languages of publication: EN

Abstracts

EN

The toxic potential of the endodontic sealers ingredients, especially the unreacted monomer, that can irritate the periapical tissue and interfere with the healing process, thus having a negative impact on the biocompatibility of the material. The aim of this study was to evaluate the influence of three experimental endodontic sealers on cells viability in vitro. Human umbilical vein endothelial cells (HUVEC) were used. The experiments were done with solid samples and extracts of sealers in artificial saliva and water. The experiments evaluated the cytotoxicity of the residual monomers that resulted from the tested composites. The decrease in cell viability was quantified by colorimetric measurement of formazan. The components of the sealers dissolved in artificial saliva and water were determined by high performance liquid chromatography (HPLC). The HUVEC are a novelty for testing the endodontic sealers biocompatibility, with certain advantages compared to other cell types used in the literature, e.g. HELA cells, fibroblasts. The data showed that cytotoxicity was directly linked with the unreacted monomer - 2-hydroxyethyl methacrylate (HEMA) present in these composites. Two of the three formulations had little or no cytotoxic effect, which makes them suitable for further testing in order to be used in endodontic treatment.

Keywords

EN

Endodontic Sealers; Cytotoxicity; Monomer Residual; Human Umbilical Vein Endothelial Cells (HUVEC)

• Publisher: De Gruyter Open
• Journal: Open Medicine
• Year: 2013
• Volume: 8
• Issue: 4
• Pages: 523-529

Dates

published

1 - 8 - 2013

online

12 - 6 - 2013

Contributors

author

Marioara Moldovan

• Department of Polymeric Composites, Babes-Bolyai University, Raluca Ripan Chemistry Research Institute, no 30 Fantanele street, Cluj-Napoca, 400294, Romania

author

Adriana Filip

• Department of Physiology, University of Medicine and Pharmacy, no 1 Clinicilor street, Cluj-Napoca, 400006, Romania

author

Cristina Prejmerean

• Department of Polymeric Composites, Babes-Bolyai University, Raluca Ripan Chemistry Research Institute, no 30 Fantanele street, Cluj-Napoca, 400294, Romania

author

Doina Prodan

• Department of Polymeric Composites, Babes-Bolyai University, Raluca Ripan Chemistry Research Institute, no 30 Fantanele street, Cluj-Napoca, 400294, Romania

author

Camelia Alb

• Faculty of Dental Medicine, University of Medicine and Pharmacy, no 8 Victor Babes street, Cluj-Napoca, 400012, Romania

author

Aniela Pop

• Faculty of General Medicine, University of Medicine and Pharmacy, no 8 Victor Babes street, Cluj-Napoca, 400012, Romania

author

Baldea Ioana

• Department of Physiology, University of Medicine and Pharmacy, no 1 Clinicilor street, Cluj-Napoca, 400006, Romania

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Identifiers

DOI

10.2478/s11536-013-0171-8