LDOC-1 and PARP-1 mRNA expression in leukocytes of father and son with cutaneous malignant melanoma

• Authors: Michele Salemi , Pier Soma , Rosita Condorelli , Domenico Recupero , Aldo Calogero
• Languages of publication: EN

Abstracts

EN

Apoptosis is central to the biology of cutaneous malignant melanoma (CMM). The leucine zipper, down regulated in cancer 1 (LDOC-1) gene, is known to be a regulator of the nuclear factor kappa B (NF-kB) through inhibition of the same NF-kB. The poly (ADP-ribose) polymerase-1 (PARP1) gene plays an important role for the efficient maintenance of genome integrity. PARP-1 protein is required for the apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus. We report here two interesting cases of family melanoma, a father and son 84 and 40 years old, respectively. The histological evaluation of the lesions of both men revealed diffused superficial melanoma with epithelioid cells. We evaluated the differential expression of LDOC-1 and PARP-1 mRNA in peripheral blood leukocytes of both the father and son. We found that both LDOC-1 and PARP-1 genes were down-regulated in both patients compared with those of controls. These data suggest that low levels of expression of LDOC-1 and PARP-1 mRNA may be associated with familial melanoma.

Keywords

EN

Cutaneous malignant melanoma; Apoptosis; LDOC-1gene; PARP-1 gene; qRT-PCR

• Publisher: De Gruyter Open
• Journal: Open Medicine
• Year: 2013
• Volume: 8
• Issue: 2
• Pages: 204-207

Dates

published

1 - 4 - 2013

online

23 - 1 - 2013

Contributors

author

Michele Salemi

• Section of Endocrinology, Andrology and Internal Medicine, Department of Internal Medicine and Systemic Diseases, University of Catania, via S. Sofia 78, 95123, Catania, Italy

author

Pier Soma

• Plastic Surgery and Burns Centre, Cannizzaro Hospital, Catania, Italy

author

Rosita Condorelli

• Section of Endocrinology, Andrology and Internal Medicine, Department of Internal Medicine and Systemic Diseases, University of Catania, via S. Sofia 78, 95123, Catania, Italy

author

Domenico Recupero

• Section of Endocrinology, Andrology and Internal Medicine, Department of Internal Medicine and Systemic Diseases, University of Catania, via S. Sofia 78, 95123, Catania, Italy

author

Aldo Calogero

• Section of Endocrinology, Andrology and Internal Medicine, Department of Internal Medicine and Systemic Diseases, University of Catania, via S. Sofia 78, 95123, Catania, Italy

References

• [1] de Vries E., Coebergh JW.: Melanoma incidence has risen in Europe, B.M.J., 2005,331, 698 http://dx.doi.org/10.1136/bmj.331.7518.698[Crossref]
• [2] Tumino R., Frasca G., Palli D., Masala G., Tagliabue G., Evangelista A., Giurdanella MC., Vineis P., Veglia F., Celentano E., Galasso R., Cascone G.: Cancer incidence in EPIC-ITALY at first follow-up, Tumori., 2003,89,656–664
• [3] Hu W., Kavanagh JJ.: Anticancer therapy targeting the apoptotic pathway, Lancet. Oncol., 2003,4, 721–729 http://dx.doi.org/10.1016/S1470-2045(03)01277-4[Crossref]
• [4] Nagasaki K., Manabe T., Hanzawa H., Maass N., Tsukada T., Yamaguchi K.: Identification of a novel gene, LDOC1, down-regulated in cancer cell lines, Cancer. Lett., 1999,140,227–234 http://dx.doi.org/10.1016/S0304-3835(99)00087-7[Crossref]
• [5] Inoue M., Takahashi K., Niide O., Shibata M., Fukuzawa M., Ra C.: LDOC1, a novel MZF-1-interacting protein, induces apoptosis, FEBS. Lett., 2005,579,604–608 http://dx.doi.org/10.1016/j.febslet.2004.12.030[Crossref]
• [6] Yu SW., Wang H., Poitras MF., Coombs C., Bowers WJ., Federoff HJ., Poirier GG., Dawson TM., Dawson VL.: Mediation of poly (ADP-ribose) polymerase-1-dependent cell death by apoptosis-inducing Factor, Science., 2002,297,259–263 http://dx.doi.org/10.1126/science.1072221[Crossref]
• [7] Yu SW., Andrabi SA., Wang H.: Apoptosis-inducing factor mediates poly (ADP-ribose) (PAR) polymerinduced cell death, Proc. Natl. Acad. Sci. USA., 2006,103,18314–18319 http://dx.doi.org/10.1073/pnas.0606528103[Crossref]
• [8] Hassa PO., and Hottiger MO.: The diverse biological roles of mammalian PARPs, a small but powerful family of poly-ADP-ribose polymerases, Front. Biosci., 2008,13,3046–3082 http://dx.doi.org/10.2741/2909[Crossref][WoS]
• [9] Livak KJ., Schmittgen TD.: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) Method, Methods., 2001,4,402–408 http://dx.doi.org/10.1006/meth.2001.1262[Crossref]
• [10] Salemi M., Giuffrida D., Soma PF., Giuffrid MC., Galia A., Calogero AE.: Two proapoptotic genes are downregulated in a patient with melanoma and repeated in-transit metastases, Am. J. Dermatopathol., 2012, 34,454–455 http://dx.doi.org/10.1097/DAD.0b013e31820a4bd7[WoS][Crossref]
• [11] Mizutani K., Koike D., Suetsugu S., Takenawa T.: WAVE3 functions as a negative regulator of LDOC1. J. Biochem, 2001,38,639–646
• [12] Nagasaki K, Schem C, von Kaisenberg C, Biallek M, Rösel F, Jonat W, Maass N.: Leucinezipper protein, LDOC1, inhibits NF-kappaB activation and sensitizes pancreatic cancer cells to apoptosis, Int. J. Cancer.,2003,105,454–458 http://dx.doi.org/10.1002/ijc.11122[Crossref]
• [13] Andrabi SA., Kim NS., Yu SW. Poly(ADP-ribose) (PAR) polymer is a death signal, Proc. Natl. Acad. Sci. USA, 2006,103,18308–18313 http://dx.doi.org/10.1073/pnas.0606526103[Crossref]

Identifiers

DOI

10.2478/s11536-012-0121-x