Adipocytes derived fibrinolytic components in peritoneum - a pilot study

• Authors: Sylvie Opatrna , Marie Korabečná , Věra Křížková , Zbynek Tonar , Jitka Kočová , Dana Mullerová
• Languages of publication: EN

Abstracts

EN

The proteins of the fibrinolytic system - urokinase plasminogen activator(uPA), tissue plasminogen activator (tPA)and plasminogen activator inhibitor type IPAI-I) - play important roles in fibrotization in various organs and including peritoneum. To study the cellular localization of PAI-1, tPA and uPA within the adipose tissue of the peritoneal membrane in patients at the onset of peritoneal dialysis(PD) we determined the initial expression of these proteins in relationship to multiple clinical variables. Methods: routinely performed parietal peritoneal biopsies in 12 patients undergoing peritoneal catheter implantation were examined. We used formalinfixed, paraffin-embedded specimens for immunohistochemical localization of these proteins along with the stereological pointcounting method for quantification of their expression within the peritoneal adipose tissue. Results: strong positive mutual correlation between the expression of PAI-1 and both uPA (SpearmanR=0.66) and tPA (R=0.59) as well as between the expression of uPA and tPA (R=0.77) was found without any relatioship to BMI, age, peritoneal transport characteristic or diabetes status. Conclusion: Adipose tissue within the peritoneum is capable of producing fibrinolysis regulators (independently on clinical parameters) thus possibly affecting the fibrotization and function of peritoneum as dialysis membrane. The effect of dialysis solution dosing, composition and other dialysis related factors should be clarified in future studies.

Keywords

EN

Peritoneal dialysis; Adipose tissue; Histology; Morphometry; PAI-1; tPA; uPA

• Publisher: De Gruyter Open
• Journal: Open Medicine
• Year: 2012
• Volume: 7
• Issue: 5
• Pages: 604-609

Dates

published

1 - 10 - 2012

online

28 - 7 - 2012

Contributors

author

Sylvie Opatrna

• Charles University Medical School and Teaching Hospital, 30166, Pilsen, Czech Republic

author

Marie Korabečná

author

Věra Křížková

• Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University in Prague, 30166, Pilsen, Czech Republic

author

Zbynek Tonar

• Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University in Prague, 30166, Pilsen, Czech Republic

author

Jitka Kočová

• Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University in Prague, 30166, Pilsen, Czech Republic

author

Dana Mullerová

• Department of Preventative Medicine and Hygiene, Faculty of Medicine in Pilsen, Charles University in Prague, 30166, Pilsen, Czech Republic

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Identifiers

DOI

10.2478/s11536-012-0042-8