Hyperhomocysteinemia’s effect on antioxidant capacity in rats

• Authors: Christian Filip , Elena Albu , Nina Zamosteanu , M. Irina , Mihaela Silion , Luminita Jerca , Nastasia Gheorghita , Mungiu Costel
• Languages of publication: EN

Abstracts

EN

Hyperhomocysteinemia represents elevated homocysteine (Hcys) concentrations in blood above the normal range. In humans, the normal range of homocysteine is 5.0–15.9 mM/ml. High levels of homocysteine disturb the normal epithelial functions and correlate with cardiovascular diseases even at slightly increased concentrations. In homocysteine metabolism, vitamins play an important role. The mechanism through which homocysteine triggers these effects is not yet elucidated, but the involvement of reactive species may be the answer. It is not known whether the intra- or extracellular antioxidant system is more affected by elevated homocysteine levels. We studied the effects of hyperhomocysteinemia on the intra- and extracellular antioxidant defense systems in two different types of diet in rats. Type I was food with low folic acid and vitamin B12 content and type II was food with normal amounts of these two vitamins. Hyperhomocysteinemia was experimentally induced by oral administration of methionine 2 mg/kg body weight, single daily dose, for a 15-day period. Plasma concentrations of homocysteine were measured using an HPLC method. In the response of the intracellular antioxidant defense system against hyperhomocysteinemia, we determined the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in red blood cells, using RANDOX kits for manual use. For the extracellular response we determined the plasma total antioxidant status (TAS) also using a RANDOX kit for manual use. Our data show that methionine load induces hyperhomocysteinemia despite normal vitamin supply in rats. SOD activity rose with simultaneous decrease in GPx activity independently of diet; this might suggest that the intracellular defense system was disturbed by the rise in homocysteine level. TAS decrease suggests that the extracellular antioxidant defense was also affected. We assume that hyperhomocysteinemia is directly linked to reactive species generation and the intracellular space seems to be more affected than the extracellular one.

Keywords

EN

Methionine; Hyperhomocysteine; TAS; SOD; GPx

• Publisher: De Gruyter Open
• Journal: Open Medicine
• Year: 2010
• Volume: 5
• Issue: 5
• Pages: 620-626

Dates

published

1 - 10 - 2010

online

20 - 8 - 2010

Contributors

author

Christian Filip

• Department of Biochemistry, Univ. Med. Pharm. “Gr. T. Popa”, 700115, Iasi, Romania

author

Elena Albu

• Department of Pharmacology and Algesiology, Univ. Med. Pharm. “Gr. T. Popa”, 700115, Iasi, Romania

author

Nina Zamosteanu

• Department of Biochemistry, Univ. Med. Pharm. “Gr. T. Popa”, 700115, Iasi, Romania

author

M. Irina

• Department of Pharmacology and Algesiology, Univ. Med. Pharm. “Gr. T. Popa”, 700115, Iasi, Romania

author

Mihaela Silion

• Laboratory of Inorganic Polymers, Inst. of Macromol. Chem. “P. Poni”, 700523, Iasi, Romania

author

Luminita Jerca

• Department of Biochemistry, Univ. Med. Pharm. “Gr. T. Popa”, 700115, Iasi, Romania

author

Nastasia Gheorghita

• Department of Biochemistry, Univ. Med. Pharm. “Gr. T. Popa”, 700115, Iasi, Romania

author

Mungiu Costel

• Department of Pharmacology and Algesiology, Univ. Med. Pharm. “Gr. T. Popa”, 700115, Iasi, Romania

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Identifiers

DOI

10.2478/s11536-010-0032-7