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Journal

2006 | 1 | 1 | 12-22

Article title

Evaluation of TV cell line viral susceptibility using conventional cell culture techniques

Content

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Languages of publication

EN

Abstracts

EN
Despite the fact that a lot of methods have been developed for rapid virus detection, classic cell culture is still “the golden standard”. The range of viruses that can be isolated and cultured in cell line systems is often limited by the susceptibility of cells to support viral replication. Since the primary cell culture, the best cellular system available to support replication of a large number of viruses, is very expensive and diffcult to obtain, cell lines, which are easier to manipulate, are commonly used for virus growth and isolation.In two previous papers we described the TV cell line initiated by our team from a laryngeal tumor, which harbors human papillomavirus (HPV) gene sequences. In this paper we analyze its capacity to support virus replication. Depending on the virus, different cytopathic effects were produced. Comparison of viral effect observed on this cell line with the effect obtained on other cell lines has been performed. This cell line might be used in the clinical virology laboratory for virus isolation.

Publisher

Journal

Year

Volume

1

Issue

1

Pages

12-22

Physical description

Dates

published
1 - 3 - 2006
online
1 - 3 - 2006

Contributors

  • “Stefan S. Nicolau” Institute of Virology, Bucharest, Romania
  • “Stefan S. Nicolau” Institute of Virology, Bucharest, Romania
author
  • “Stefan S. Nicolau” Institute of Virology, Bucharest, Romania
author
  • “Stefan S. Nicolau” Institute of Virology, Bucharest, Romania
author
  • “Stefan S. Nicolau” Institute of Virology, Bucharest, Romania
  • “Stefan S. Nicolau” Institute of Virology, Bucharest, Romania

References

  • [1] Mutiu, I. Alexiu, M. Chivu, M. Petica, G. Anton, C. Bleotu, C.C. Diaconu, C. Popescu, V. Jucu and C. Cernescu: “Detection of human papillomavirus gene sequences in cell lines derived from laryngeal tumours”, J. Cell. Mol. Med. Vol. 5, (2001), pp. 49–59.
  • [2] C.C. Diaconu, C. Bleotu, M. Chivu, I. Alexiu, D. Petrusca, G. Anton, R. Achim, S.M. Ruta and C.C. Cernescu: “The development of larger cells that spontaneously escape senescence - a step during the immortalization of a human cancer cell line”, J. Cell. Mol. Med., Vol. 8, (2004), pp. 93–101.
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  • [9] S.M. Lipson, K. David, F. Shaikh and L. Qian: “Detection of precytopathic effect of enteroviruses in clinical specimens by centrifugation-enhanced antigen detection”, JCM Vol. 39, (2001), pp. 2755–2759. [PubMed]
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  • [11] J. Ma, C.P. Gerba and I.L. Pepper: “Increased sensitivity of poliovirus detection in tap water concentrates by reverse transcriptase-polymerase chain reaction”, J. Virol. Methods Vol. 55, (1995), pp. 295–302. http://dx.doi.org/10.1016/0166-0934(95)00065-6[Crossref]
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Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.-psjd-doi-10_2478_s11536-006-0007-x
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