Chemometric analysis of volatiles of propolis from different regions using static headspace GC-MS

• Authors: Vilma Kaškonienė , Paulius Kaškonas , Audrius Maruška , Loreta Kubilienė
• Languages of publication: EN

Abstracts

EN

Six samples of propolis were analyzed in the paper: a sample from Brazil, Estonia, China and three samples from different locations of Uruguay. Static headspace technique coupled with gas chromatography-mass spectrometry analysis has been applied for the determination of the characteristic volatile profile with the aim to differentiate the propolis from different regions. Monoterpenes (α- and β-pinenes) were predominant in all samples, except the sample from China. This sample separated itself by the alcohols 3-methyl-3-buten-1-ol and 3-methyl-2-buten-1-ol, (40.33% and 11.57%, respectively) and ester 4-penten-1-yl acetate (9.04%). α-Pinene and β-pinene composed 64.59–77.56% of volatiles in Brazilian and Uruguayan propolis, and 29.43% in Estonian propolis. Brazilian propolis was distinguished by a high amount of β-methyl crotonaldehyde (10.11%), one of Uruguayan samples 3- by limonene (15.58%), and the Estonian sample - by eucalyptol (25.95%). Statistical investigation of the samples was made applying principal component, hierarchical cluster and K-Means cluster analyses. Various data pre-processing techniques were proposed and used to study and obtain the important volatile compounds contributed to the differentiation of the propolis samples from different regions to separate clusters.

Keywords

EN

Propolis; Static headspace GC-MS; Data pre-processing; Hierarchical cluster analysis; K-Means cluster analysis

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2014
• Volume: 12
• Issue: 6
• Pages: 736-746

Dates

published

1 - 6 - 2014

online

13 - 3 - 2014

Contributors

author

Vilma Kaškonienė

• Vytautas Magnus University

author

Paulius Kaškonas

• Kaunas University of Technology

author

Audrius Maruška

• Vytautas Magnus University

author

Loreta Kubilienė

• Lithuanian University of Health Science

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Identifiers

DOI

10.2478/s11532-014-0521-7