UV-absorption studies of interaction of karanjin and karanjachromene with ds. DNA: Evaluation of binding and antioxidant activity

• Authors: Nasima Arshad , Naghmana Rashid , Sajida Absar , Muhammad Abbasi , Samreen Saleem , Bushra Mirza
• Languages of publication: EN

Abstracts

EN

Two flavonoids, karanjin (Kj) and karanjachromene (Kc) have been investigated spectrophotometrically for their mode of interactions with double stranded (ds)-DNA at blood (7.4) and stomach (4.7) pH and at human body temperature (37°C). Benesi-Hildebrand equation was used to evaluate the binding constants, K b . Binding constants at both pH values and at body temperature showed stronger binding of both the flavonoids and formation of 1:1 flavonoid-DNA complex via intercalative mode. However, K b values for karanjin were evaluated to be comparatively greater than karanjachromene at both pH values. The highest value of binding constant (1.32×105 M−1) for karanjin at blood pH (7.4) demonstrated its comparatively stronger binding and greater effectiveness at this pH. Standard Gibbs free energy changes (ΔG) of flavonoid-DNA complexes were calculated as negative values and indicative of spontaneity of their binding. Both flavonoids showed significant DNA protection activity. [...]

Keywords

EN

Flavonoids; UV-Vis spectroscopy; Interaction with chicken blood ds.DNA; Binding constants; Antioxidant activity

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2013
• Volume: 11
• Issue: 12
• Pages: 2040-2047

Dates

published

1 - 12 - 2013

online

26 - 9 - 2013

Contributors

author

Nasima Arshad

• Department of Chemistry, Allama Iqbal Open University, Islamabad, 44000, Pakistan

author

Naghmana Rashid

• Department of Chemistry, Allama Iqbal Open University, Islamabad, 44000, Pakistan

author

Sajida Absar

• Department of Chemistry, Allama Iqbal Open University, Islamabad, 44000, Pakistan

author

Muhammad Abbasi

• Department of Chemistry, Allama Iqbal Open University, Islamabad, 44000, Pakistan

author

Samreen Saleem

• Department of Biochemistry, Quaid-i-Azam University, Islamabad, 45320, Pakistan

author

Bushra Mirza

• Department of Biochemistry, Quaid-i-Azam University, Islamabad, 45320, Pakistan

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Identifiers

DOI

10.2478/s11532-013-0327-z