High resolution mass spectrometric characterization of amino linked oligosaccharides - a preliminary study

• Authors: Roxana Ghiulai , Mirela Galusca , Ioana Sisu , Eugen Sisu , Alina Zamfir
• Languages of publication: EN

Abstracts

EN

In this study maltose, maltotriose and maltotetraose were for the first time, coupled to 4,4′-methylenedianiline (MDA). The aim of this preliminary work was to test the feasibility of oligo- and polysaccharide coupling to MDA and the characterization of the coupling products by high resolution mass spectrometry (MS). (+) nanoESI in combination with a quadrupole time of flight (QTOF) MS in full scan (MS) and MS/MS was optimized first on underivatized maltose, maltotriose and maltotetraose. The optimal screening and sequencing conditions were further applied to the MDA-functionalized oligosaccharides. The obtained results revealed a straightforward MS detection of the functionalized oligomers, high sequence coverage and a fragmentation pathway with the formation of B and Y ions as well as the complementary C and Z ions along with a typical cleavage of the aglycon. We consider that this methodology is fully applicable also to polydisperse mixtures of long chain polysaccharides, which due to the large number of components and their size require a systematic method of development and testing. [...]

Keywords

EN

MDA-functionalized oligosaccharides; Nanoelectrospray; QTOF MS; CID MS/MS

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2013
• Volume: 11
• Issue: 8
• Pages: 1309-1319

Dates

published

1 - 8 - 2013

online

1 - 6 - 2013

Contributors

author

Roxana Ghiulai

• Department of Biochemistry, University of Medicine and Pharmacy “Victor Babes”, 300041, Timisoara, Romania

author

Mirela Galusca

author

Ioana Sisu

• Department of Biochemistry, University of Medicine and Pharmacy “Victor Babes”, 300041, Timisoara, Romania

author

Eugen Sisu

• Department of Biochemistry, University of Medicine and Pharmacy “Victor Babes”, 300041, Timisoara, Romania

author

Alina Zamfir

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Identifiers

DOI

10.2478/s11532-013-0265-9