Rapid flow injection electrochemical detection of 3,3′,4,4′ tetrachlorobiphenyl using stabilized lipid membranes with incorporated sheep antibody

• Authors: Dimitrios Nikolelis , Nikolas Psaroudakis , Antonis Michaloliakos , Georgia-Paraskevi Nikoleli , Michael Scoullos
• Languages of publication: EN

Abstracts

EN

An electrochemical biosensor based on a supported polymerized lipid film with incorporated sheep anti-3,3′,4,4′ tetrachlorobiphenyl (PCB congener 77) antibody using flow injection analysis was developed. The polymerized lipid film contained 85% (w/w) dipalmitoylphosphatidylcholine (DPPC) and 15% (w/w) dipalmitoylphosphatidic acid (DPPA), methacrylic acid, ethylene glycol dimethacrylate, AIBN and sheep anti-congener 77 antiserum. Congener 77 was injected into flowing carrier electrolyte and the flow stopped to detect the antigen. These membranes gave only a single transient proportional to log [congener 77] from 10−8 to 10−5 M, with a detection limit of ca. 10−8 M. A membrane containing 35% (w/w) DPPA was used to examine regeneration. The maximum number of cycles was about 5. [...]

Keywords

EN

Congener 77; Stabilized lipid films; Biosensor; Flow injection; Antibody regeneration

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2013
• Volume: 11
• Issue: 2
• Pages: 320-323

Dates

published

1 - 2 - 2013

online

29 - 11 - 2012

Contributors

author

Dimitrios Nikolelis

• Laboratory of Environmental Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, GR-15771, Athens, Greece

author

Nikolas Psaroudakis

• Laboratory of Inorganic Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, GR-15771, Athens, Greece

author

Antonis Michaloliakos

• Laboratory of Environmental Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, GR-15771, Athens, Greece

author

Georgia-Paraskevi Nikoleli

• Laboratory of Inorganic & Analytical Chemistry, School of Chemical Engineering, Dept 1, Chemical Sciences, National Technical University of Athens, GR-15780, Athens, Greece

author

Michael Scoullos

• Laboratory of Environmental Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis-Kouponia, GR-15771, Athens, Greece

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Identifiers

DOI

10.2478/s11532-012-0157-4