Fully automated chip-based nanoelectrospray combined with electron transfer dissociation for high throughput top-down proteomics

• Authors: Corina Flangea , Catalin Schiopu , Florina Capitan , Cristina Mosoarca , Marilena Manea , Eugen Sisu , Alina Zamfir
• Languages of publication: EN

Abstracts

EN

The conventional protocol for protein identification by electrospray ionization mass spectrometry (MS) is based on enzymatic digestion which renders peptides to be analyzed by liquid chromatography-MS and collision-induced dissociation (CID) multistage MS, in the so-called bottom-up approach. Though this method has brought a significant progress to the field, many limitations, among which, the low throughput and impossibility to characterize in detail posttranslational modifications in terms of site(s) and structure, were reported. Therefore, the research is presently focused on the development of procedures for efficient top-down fragmentation of intact protein ions. In this context, we developed here an approach combining fully automated chip-based-nanoelectrospray ionisation (nanoESI), performed on a NanoMate robot, with electron transfer dissociation (ETD) for peptide and top-down protein sequencing and identification. This advanced analytical platform, integrating robotics, microfluidics technology, ETD and alternate ETD/CID, was tested and found ideally suitable for structural investigation of peptides and modified/functionalized peptides as well as for top-down analysis of medium size proteins by tandem MS experiments of significantly increased throughput and sensitivity. The obtained results indicate that NanoMate-ETD and ETD/CID may represent a viable alternative to the current MS strategies, with potential to develop into a method of routine use for high throughput top-down proteomics.

Keywords

EN

Chip-electrospray mass spectrometry; Electron transfer dissociation; Peptides; Proteins; Top-down analysis

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2013
• Volume: 11
• Issue: 1
• Pages: 25-34

Dates

published

1 - 1 - 2013

online

24 - 10 - 2012

Contributors

author

Corina Flangea

• “Aurel Vlaicu” University of Arad

author

Catalin Schiopu

• “Aurel Vlaicu” University of Arad

author

Florina Capitan

• “Aurel Vlaicu” University of Arad

author

Cristina Mosoarca

author

Marilena Manea

• University of Konstanz

author

Eugen Sisu

• Chemistry Institute of Romanian Academy

author

Alina Zamfir

• “Aurel Vlaicu” University of Arad

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Identifiers

DOI

10.2478/s11532-012-0130-2