Use of multivariate statistical techniques to optimize the separation of isoflavones by liquid chromatography

• Authors: Irena Baranowska , Sylwia Magiera , Jacek Baranowski
• Languages of publication: EN

Abstracts

EN

The aim of this paper is to optimize and validate a high performance liquid chromatography (HPLC) method for separation and quantification of five isoflavones. A statistical central composite design was used to separate all peaks. These multivariate procedures were efficient in determining the optimal separation condition using resolution, capacity factor, asymmetry and number of theoretical plates. [...] The effective separation of the examined compounds was applied on a Develosil RP Aqueous AR 5 RP-30 column with a gradient mobile phase system and a DAD detector. The isolation and preconcentration of the isoflavones from urine and plasma samples were conducted by means of the solid-phase extraction (SPE). For optimize SPE conditions various sorbents were tested. Furthermore, high recoveries and good relative standard deviations were obtained when the samples were passed through the Oasis HLB column. The developed method was validated and successfully applied for determination of isoflavones in urine and plasma.

Keywords

EN

Central composite design; Isoflavones; HPLC; Solid-phase extraction

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2011
• Volume: 9
• Issue: 6
• Pages: 972-981

Dates

published

1 - 12 - 2011

online

27 - 9 - 2011

Contributors

author

Irena Baranowska

• Department of Analytical Chemistry, Chemical Faculty, Silesian University of Technology, 44-100, Gliwice, Poland

author

Sylwia Magiera

• Department of Analytical Chemistry, Chemical Faculty, Silesian University of Technology, 44-100, Gliwice, Poland

author

Jacek Baranowski

• Department of Clinical Physiology, University Hospital, SE-581 85, Linköping, Sweden

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Identifiers

DOI

10.2478/s11532-011-0093-8