Preparation of polymer based sorbents for solid phase extraction of polyphenolic compounds

• Authors: Muhammad Qureshi , Guenther Stecher , Christian Huck , Guenther Bonn
• Languages of publication: EN

Abstracts

EN

This article discusses the preparation of different polymeric sorbents for solid phase extraction. Various monomers like acrylamide, methacrylic acid and 4-vinylpyridine (VP), cross-linkers such as divinylbenzene (DVB) and ethyleneglycoldimethacrylate (EDMA), porogens like tetrahydrofuran (THF) and dimethylsulphoxide (DMSO) / acetonitrile (ACN) with different ratios were investigated in order to optimize recoveries. Resulting polymers were characterized through scanning electron microscope (SEM) and compared with Oasis HLB (Waters, MA, USA) and Strata-X (Phenomenex, Torrance, USA) on the basis of extraction performance, recovery efficiency and loading capacity. Sample applied was a mixture of flavonoid standards (rutin, myricetin, luteolin, quercetin, apigenin and kaempferol). HPLC hyphenated with PDA was used for the analysis of samples. Results showed that among the prepared SPE materials, 4-VP-co-EDMA produced best results. Comparison of the produced polymers with of Oasis HLB and Strata-X resulted in comparable efficiencies; especially the polymer 4-VP-co-EDMA gave almost similar results for all analytes to those of commercially available SPE materials. A general trend of decrease in retention efficiency with increase in polarity has been observed in both synthesized and already available SPE materials. The newly synthesized polymeric materials can be employed as SPE sorbents for efficient extraction of polyphenolic compounds especially for flavonoid aglycons.

Keywords

EN

Solid phase extraction; Flavonoid standard mixture; Polymer sorbents; Oasis HLB; Strata-X

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2011
• Volume: 9
• Issue: 2
• Pages: 206-212

Dates

published

1 - 4 - 2011

online

17 - 2 - 2011

Contributors

author

Muhammad Qureshi

author

Guenther Stecher

author

Christian Huck

• Leopold-Franzens-University Innsbruck

author

Guenther Bonn

• Leopold-Franzens-University Innsbruck

References

• [1] J.L. Luque-Garcia, M.D. Luque de Castro, Trends Anal. Chem. 23, 102 (2004) http://dx.doi.org/10.1016/S0165-9936(04)00302-4[Crossref]
• [2] C.W. Huck, G.K. Bonn, J. Chromatogr. A, 885, 51 (2000) http://dx.doi.org/10.1016/S0021-9673(00)00333-2[Crossref]
• [3] M.C. Hennion, J. Chromatogr. A, 856, 3 (1999) http://dx.doi.org/10.1016/S0021-9673(99)00832-8[Crossref]
• [4] N.J.K. Simpson (Ed.), Solid-phase extraction: principles, techniques, and applications (Marcel Dekker Inc., New York, 2000)
• [5] C.F. Poole, Trends Anal. Chem. 22, 362 (2003) http://dx.doi.org/10.1016/S0165-9936(03)00605-8[Crossref]
• [6] E. Blahová, E. Brandšteterová, Chem. Pap. 58, 362 (2004)
• [7] M.J. Hilton, K.V. Thomas, J. Chromatogr. A, 1015, 129 (2003) http://dx.doi.org/10.1016/S0021-9673(03)01213-5[Crossref]
• [8] A.A. D’Archivio, M. Fanelli, P. Mazzeo, F. Ruggieri, Talanta, 71, 25 (2007) http://dx.doi.org/10.1016/j.talanta.2006.03.016[Crossref]
• [9] M.D. Marazuela, M.C. Moreno-Bondi, J. Chromatogr. A, 1034, 25 (2004) http://dx.doi.org/10.1016/j.chroma.2004.02.022[Crossref]
• [10] J. Xie, L. Zhu, H. Luo, L. Zhou, C. Li, X. Xu, J. Chromatogr. A, 934, 1 (2001) http://dx.doi.org/10.1016/S0021-9673(01)01294-8[Crossref]
• [11] P.V. Prabhakaran, S. Venkatachalam, K.N. Ninan, Eur. Polym. J. 35, 1743 (1999) http://dx.doi.org/10.1016/S0014-3057(98)00246-8[Crossref]
• [12] G. Theodoridis, M. Lasakova, V. Skerikova, A. Tegou, N. Giantsiou, P. Jandera, J. Sep. Sci. 29, 2310 (2006) http://dx.doi.org/10.1002/jssc.200500492[Crossref]
• [13] J. O’Mahony, A. Molinelli, K. Nolan, M.R. Smyth, B. Mizaikoff, Biosens. and Bioelectron. 21, 1383 (2006) http://dx.doi.org/10.1016/j.bios.2005.05.015[Crossref]
• [14] A. Molinelli, R. Weiss, B. Mizaikoff, J. Agric. Food Chem. 50, 1804 (2002) http://dx.doi.org/10.1021/jf011213q[Crossref]
• [15] G. Stecher, C.W. Huck, M. Popp, G.K. Bonn, Fresenius J. Anal. Chem. 371, 73 (2001) http://dx.doi.org/10.1007/s002160100898[Crossref]
• [16] N. Masque, R.M. Marce, F. Borrull, Trends Anal. Chem. 17, 384 (1998) http://dx.doi.org/10.1016/S0165-9936(98)00019-3[Crossref]

Identifiers

DOI

10.2478/s11532-011-0006-x