Screen-printed electrodes modified with HRP-zirconium alcoxide film for the development of a biosensor for acetaminophen detection

• Authors: Veronica Sima , Cecilia Cristea , Ede Bodoki , Gabriela Duţu , Robert Sāndulescu
• Languages of publication: EN

Abstracts

EN

The development of a biosensor based on the immobilization of horseradish peroxidase (HRP) within a zirconium alkoxide-polyetilenimine film onto screen-printed electrodes (SPE) for acetaminophen detection and acetaminophen quantification in pharmaceutical products is described. The biosensor operation mode is based on monitoring the amperometric signal produced by the electrochemical reduction of the enzymatically generated electroactive oxidized species of acetaminophen in the presence of hydrogen peroxide. The enzyme immobilization is performed by retention in a polyethylenimine-zirconium alcoxide porous gel film, a technique that offers good entrapping and a protective environment for the biocomponent due to the hydration properties of the immobilization layer. SPEs have the advantage of being easily mass-produced at low costs with superior characteristics in comparison with classical electrode materials. In this configuration, zirconium alkoxide demonstrates its electrocatalytic activity. The biosensor allows the quantification of acetaminophen with a limit of detection of 6.21×10−8 M and a linear range between 4.35×10−7 M and 4.98×10−6 M. Finally, the biosensor is applied to the quantitative analysis of acetaminophen in Perdolan® tablets. [...]

Keywords

EN

Electrochemical biosensor; Horseradish peroxidase; Zirconium alkoxide; SPE; Acetaminophen

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2010
• Volume: 8
• Issue: 5
• Pages: 1034-1040

Dates

published

1 - 10 - 2010

online

5 - 9 - 2010

Contributors

author

Veronica Sima

• Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania

author

Cecilia Cristea

• Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania

author

Ede Bodoki

• Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania

author

Gabriela Duţu

• Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania

author

Robert Sāndulescu

• Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania

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Identifiers

DOI

10.2478/s11532-010-0070-7