Qualitative and quantitative analysis of gallic acid in Alchemilla vulgaris, Allium ursinum, Acorus calamus and Solidago virga-aurea by chip-electrospray ionization mass spectrometry and high performance liquid chromatography

• Authors: Dumitru Condrat , Cristina Mosoarca , Alina Zamfir , Florin Crişan , Maria Szabo , Alfa Lupea
• Languages of publication: EN

Abstracts

EN

This study presents the results obtained from qualitative and quantitative analysis of gallic acid from hydro-alcoholic extracts (methanol, ethanol) of plants from Plantae regnum. Plant qualitative analysis was performed using a novel mass spectrometric (MS) method based on fully automated chip-nanoelectrospray ionization (nanoESI) high capacity ion trap (HCT) while quantitative analysis was carried out by high performance liquid chromatography (HPLC). These methods were applied to Alchemilla vulgaris - common lady’s-mantle (aerial part), Allium ursinum - bear’s garlic (leaves), Acorus calamus - common sweet flag (roots), Solidago virga-aurea - goldenrod (aerial part). Obtained results indicated that methanol extracts (96%, 80%) have a gallic acid content ranging between 0.0011–0.0576 mg mL−1 extract while the ethanol extracts (96%, 60%) exhibit a gallic acid concentration that varies between 0.0010–0.0182 mg mL−1 extract. [...]

Keywords

EN

Gallic acid; HPLC; Chip-ESI MS; Hydro-alcoholic extracts; Plantae

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2010
• Volume: 8
• Issue: 3
• Pages: 530-535

Dates

published

1 - 6 - 2010

online

25 - 4 - 2010

Contributors

author

Dumitru Condrat

• Department of Chemical and Biological Sciences, Aurel Vlaicu University, Arad, 310330, Romania

author

Cristina Mosoarca

• National Institute for Research and Development in Electrochemistry and Condensed Matter, Timisoara, 300224, Romania

author

Alina Zamfir

author

Florin Crişan

• County Lab for Pesticides Quality Control, Arad, 310059, Romania

author

Maria Szabo

• Department of Chemical and Biological Sciences, Aurel Vlaicu University, Arad, 310330, Romania

author

Alfa Lupea

• Faculty of Industrial Chemistry and Environmental Engineering, Politehnica University, Timişoara, 300006, Romania

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Identifiers

DOI

10.2478/s11532-010-0012-4