Comparison of pressurised fluid and ultrasonic extraction methods for analysis of plant antioxidants and their antioxidant capacity

• Authors: Petr Dobiáš , Petra Pavlíková , Martin Adam , Aleš Eisner , Blanka Beňová , Karel Ventura
• Languages of publication: EN

Abstracts

EN

The analytical method based on the high-performance liquid chromatography coupled with UV detection (HPLC/UV) for determination of selected antioxidants (i.e., esculetin, scopoletin, 7-hydroxycoumarine, rutin, xanthotoxin, 5-methoxypsoralen and quercetin) in plant material was developed. Pressurised fluid extraction (PFE) and ultrasonic extraction (USE) methods for the isolation of these compounds from ten real plant samples were used. Both extraction methods were optimised and compared to each other. For the proposed HPLC/UV method the LOQ values (limit of quantification) in the range from 22.7 (xanthotoxin) to 97.2 ng mL−1 (rutin) were obtained. For all extracts the antioxidant capacity based on the reduction of free 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) was also determined. Results ranged from 82.04 to 94.43% of DPPH radical inhibition for PFE method and from 76.01 to 89.94% in the case of USE method. [...]

Keywords

EN

Pressurised Fluid Extraction; Ultrasonic Extraction; Antioxidants; Antioxidant Capacity; High-Performance Liquid Chromatography

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2010
• Volume: 8
• Issue: 1
• Pages: 87-95

Dates

published

1 - 2 - 2010

online

16 - 2 - 2010

Contributors

author

Petr Dobiáš

• Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic

author

Petra Pavlíková

• Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic

author

Martin Adam

• Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic

author

Aleš Eisner

• Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic

author

Blanka Beňová

• Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic

author

Karel Ventura

• Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic

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Identifiers

DOI

10.2478/s11532-009-0125-9