Simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with 7,12-dimethylbenz[a]anthracene and Hypericum Perforatum L. by high-performance liquid chromatography with diode-array detection

• Authors: Abdulkadir Levent , Suat Ekin , Gökhan Oto
• Languages of publication: EN

Abstracts

EN

A new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with Hypericum Perforatum L. and 7,12-dimethylbenz[a]anthracene. Furthermore, vitamin C was determined spectrophotometrically. High-performance liquid chromatography analysis was performed utilizing an Inertsil ODS3 reversed phase column with methanol-acetonitrile-tetrahydrofuran (65:30:5, v/v/v) as mobile phase, at a flow rate of 1.5 mL min−1 and 40°C. Diode-array detection was conducted at 325 and 450 nm for retinol and retinyl palmitate, and β-carotene, respectively with a running time of 26 min. The high-performance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. This method was then applied to determine the amounts of retinol, retinyl palmitate and β-carotene in rat serum. Results of this study demonstrated that at 60th day in the 7,12-dimethylbenz[a]anthracene-treated group there was a significant decrease (pa] anthracene + Hypericum Perforatum L. treated group compared to the control group.. [...]

Keywords

EN

Retinol; HPLC; Hypericum Perforatum L.; 7,12-dimethylbenz[a]anthracene

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2010
• Volume: 8
• Issue: 1
• Pages: 108-115

Dates

published

1 - 2 - 2010

online

16 - 2 - 2010

Contributors

author

Abdulkadir Levent

• Department of Analytical Chemistry, Faculty of Arts and Sciences, Yuzuncu Yil University, 65080, Van, Turkey

author

Suat Ekin

• Department of Biochemistry, Faculty of Arts and Sciences, Yuzuncu Yil University, 65080, Van, Turkey

author

Gökhan Oto

• Department of Pharmacology, Medical Faculty, Yuzuncu Yil University, 65080, Van, Turkey

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Identifiers

DOI

10.2478/s11532-009-0101-4