Potential applications of fluorhydroxyapatite as biomaterials in medicine

• Authors: Martin Vitkovič , Maha Noaman , Martin Palou , Soňa Jantová
• Languages of publication: EN

Abstracts

EN

The present work was undertaken to investigate the bioactivity and cytotoxicity of fluorhydroxyapatite ceramics. The bioactivity was evaluated by in vitro testing in simulated body fluid (SBF), in which ion concentrations are almost identical with inorganic ion concentrations of human blood plasma. Pellets of FA, HA and FHA were immersed in SBF for 48 hours, 1 week and 4 weeks at 36.5°C. Changes of the surface microstructure of the samples were observed by scanning electron microscopy (SEM). 48 hours and one week immersion in SBF did not result in any substantial progress in bioactivity. After 4 weeks in SBF a new biologically active layer was created on the surface of the biomaterials. In addition, the embryonal mouse fibroblast cell line NIH-3T3 was used for a comparative study of basal cytotoxicity of FHA, HA and FA discs. The sensitivity of these cells for tested biomaterials was evaluated on the basis of two cytotoxic end points: cell proliferation and cell morphology. The basal cytotoxicity of FHA, FA and HA discs was measured by a direct contact method. After 24, 48 and 72 hours, the cell growth was evaluated by direct counting of non-affected cells and cells treated by biomaterials. After 72 hours of biomaterials treatment, about 25% inhibition of cell number and unchanged morphology was found. [...]

Keywords

EN

Fluorhydroxyapatite ceramics; Biomaterials; Bioactivity; Cytotoxicity

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2009
• Volume: 7
• Issue: 2
• Pages: 246-251

Dates

published

1 - 6 - 2009

online

9 - 4 - 2009

Contributors

author

Martin Vitkovič

• Slovak University of Technology, Faculty of Chemical and Food Technology, 812 37, Bratislava, Slovak Republic

author

Maha Noaman

• Slovak University of Technology, Faculty of Chemical and Food Technology, 812 37, Bratislava, Slovak Republic

author

Martin Palou

• Slovak University of Technology, Faculty of Chemical and Food Technology, 812 37, Bratislava, Slovak Republic

author

Soňa Jantová

• Slovak University of Technology, Faculty of Chemical and Food Technology, 812 37, Bratislava, Slovak Republic

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Identifiers

DOI

10.2478/s11532-009-0010-6