Spectroscopic methods of studying enzyme action in wool fibre

• Authors: Magdalena Fogorasi , Elisabeth Heine
• Languages of publication: EN

Abstracts

EN

Enzymes may be used to develop an environmentally friendly alternative to the conventional polluting technologies in textile finishing. The action of the unlabeled and fluorescent labeled proteolytic enzymes subtilisin and trypsin on wool was examined. Scanning electron micrographs and a diffusion study, based on fluorescence microscopy, localized the enzymatic attack on the fiber. A kinetic study was carried out by monitoring the amino acid content of the treatment liquor.Enzymatic action is not confined to the fiber surface. To limit attack to the surface and reduce wool damage new treatment methods such as enzyme immobilization onto a solid carrier must be investigated.

Keywords

EN

Proteolytic activity; diffusion; kinetic measurements; fluorescence microscopy; wool; subtilisin; trypsin; fiber finishing

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2006
• Volume: 4
• Issue: 4
• Pages: 786-797

Dates

published

1 - 12 - 2006

online

1 - 12 - 2006

Contributors

author

Magdalena Fogorasi

• Textile Department, Aurel Vlaicu University, 310130, Arad, Romania

author

Elisabeth Heine

• Deutsches Wollforschungsinstitut an der RWTH Aachen e. V., D-52056, Aachen, Germany

References

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Identifiers

DOI

10.2478/s11532-006-0045-x