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2015 | 124 | 1 | 38-41

Article title

The gene expression of class III inhibitors of apoptosis in arteriosclerotic disease

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Languages of publication

EN

Abstracts

EN
Introduction. Recent research shows that programmed cell death has great importance in the pathomechanism of atherosclerosis. The BIRC5 and BIRC6 genes belong to Class III IAPs with the anti-apoptotic effect. The proteins display multidirectional action. According to the available literature, in addition to the effect of apoptosis inhibition they also display other properties. It is suggested that they play an important role in the processes of proliferation and cellular differentiation. Aim. The aim of the study was to assess the expression of the BIRC5 and BIRC6 genes in normal peripheral blood lymphocytes and in peripheral blood lymphocytes of patients diagnosed with atherosclerosis. Material and methods. The analysis was carried out on RNA samples obtained from peripheral blood lymphocytes of 21 patients with diagnosed atherosclerosis. The specific fragment of the analysed gene was obtained through amplification with the use of cDNA synthesised in the reaction of reverse transcription. The test of expression was conducted with the use of the Real-Time PCR method. In the studied cases, the level of expression of the analysed gene was compared to the level of expression of the reference gene, B2M. Results. The study showed that mRNA of the BIRC5 and BIRC6 genes is present in the cells of patients with atherosclerosis, as well as in the cells of healthy individuals. The cells taken from the patients with atherosclerosis were mainly characterized by an increased gene expression in comparison to the normal cells. Conclusion. Increased BIRC6 and BIRC5 gene expression in the cells of the patients with atherosclerosis can suggest an increased amount of the inhibitor protein BRUCE and survivin, and also decreased sensitivity of cells to apoptosis. In the case of the patients who had significantly higher expression of the BIRC6 gene in lymphocytes compared to the norm, hypertension and diabetes mellitus were more common

Keywords

Publisher

Year

Volume

124

Issue

1

Pages

38-41

Physical description

Dates

published
1 - 3 - 2014
online
23 - 4 - 2014

Contributors

  • Department of Clinical Genetics, Medical University of Lublin, Poland
  • Department of Clinical Genetics, Medical University of Lublin, Poland
author
  • Chair and Department of Vascular Surgery and Angiology, Medical University of Lublin, Poland
  • Department of Clinical Genetics, Medical University of Lublin, Poland
author
  • Department of Clinical Genetics, Medical University of Lublin, Poland
author
  • Department of Clinical Genetics, Medical University of Lublin, Poland
author
  • Department of Clinical Genetics, Medical University of Lublin, Poland
  • Department of Clinical Genetics, Medical University of Lublin, Poland
  • Department of Clinical Genetics, Medical University of Lublin, Poland
  • Chair and Department of Vascular Surgery and Angiology, Medical University of Lublin, Poland
  • Chair and Department of Pharmaceutical Botany, Medical University of Lublin, Poland
author
  • Department of Clinical Genetics, Medical University of Lublin, Poland 11 Radziwiłłowska Str., 20-080 Lublin, Poland tel: +48 81 528-84-08, fax: +48 81 528-84-08

References

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  • 15. Deveraux QL, Reed JC. IAP family proteins suppressor of apoptosis. Genes Dev. 1999;13:239-52.
  • 16. Bielak-Żmijewska A. Mechanizmy oporności komórek nowotworowych na apoptozę. Kosmos. Problemy Nauk Biologicznych. 2003;52:157-71.
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  • 19. Grzybowska-Izydorczyk O, Smolewski P. Białka inhibitorowe apoptozy z rodziny inhibitorów apoptozy (IAP) i ich antagoniści: rola biologiczna oraz potencjalne znaczenie w karcinogenezie i celowanej terapii przeciwnowotworowej. Acta Haematol Pol. 2009;40:593-612.
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  • 21. Kaczmarek-Borowska B, Zmorzyński Sz, Filip A. Biologiczna rola surwiwiny. Współ Onkol. 2008;10:437-40.
  • 22. Rupniewska Z, Koczkodaj D, Wąsik-Szczepanek E. Ekspresja surwiwiny i jej znaczenie kliniczne (Część II). Acta Haematol Pol. 2005;36:381-7.
  • 23. Yamamoto K, Abe S, Nakagawa Y, et al. Expression of IAP proteins in myelodysplastic syndromes trans forming to overt leukemia. Leuk Res. 2004;28:1203-11.[Crossref]
  • 24. Sokołowska J, Urbańska K. Biologiczna rola surwiwiny i jej znaczenie kliniczne. Życie Wet. 2011;86:963-6.
  • 25. Hansson GK, Libby P. The immune response in atherosclerosis: a double-edged sword. Nat Rev Immunol. 2006;6:508-19. Acknowledgements. This study was supported in part by founds from: Project “The equipment of innovative laboratories doing research on new medicines used in the therapy of civilization and neoplastic diseases” within the Operational Program Development of Eastern Poland 2007-2013, Priority Axis I Modern Economy, Operations I.3 Innovation Promotion and Projects: The “Study grants for PhD II” co-financed by the European Social Fund of the State Budget and Budget Lublin under Human Capital Operational Programme Priority VIII Regional human resources, Measure 8.2 Transfer of knowledge, Measure 8.2.2 Regional Innovation Strategies and The “Expertise and Competence” - the development of the Medical University of Lublin “co-financed by the European Social Fund within the Human Capital Operational Programme 2007-2013

Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.-psjd-doi-10_2478_pjph-2014-0008
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