Investigation of the toxicity of the ionic liquid 1-butyl-3-methylimidazolium chloride to Saccharomyces cerevisiae AY93161 for lignocellulosic ethanol production

• Authors: Shengdong Zhu , Pei Yu , Mingke Lei , Yanjie Tong , Lu Zheng , Rui Zhang , Jun Ji , Qiming Chen , Yuanxin Wu
• Languages of publication: EN

Abstracts

EN

Ionic liquid (IL) pretreatment of lignocellulosic materials has provided a new technical tool to improve lignocellulosic ethanol production. To evaluate the influence of the residual IL in the fermentable sugars from enzymatic hydrolysis of IL pretreatment of lignocellulosic materials on the subsequent ethanol fermentation, the toxicity of the IL 1-butyl-3-methylimidazolium chloride ([BMIM]Cl) to Saccharomyces cerevisiae AY93161 was investigated. Firstly, the morphological structure, budding and metabolic activity of Saccharomyces cerevisiae AY93161 at different [BMIM]Cl concentrations were observed under an optical microscope. The results show that its single cell morphology remained unchanged at all [BMIM]Cl concentrations, but its reproduction rate by budding and its metabolic activity decreased with the [BMIM]Cl concentration increasing. The half effective concentration (EC50) and the half inhibition concentration (IC50) of [BMIM]Cl to Saccharomyces cerevisiae AY93161 were then measured using solid and liquid suspension culture and their value were 0.53 and 0.39 g.L-1 respectively. Finally, the influence of [BMIM]Cl on ethanol production was investigated. The results indicate that the [BMIM]Cl inhibited the growth and ethanol production of Saccharomyces cerevisiae AY93161. This toxicity study provides useful basic data for further development in lignocellulosic ethanol production by using IL technology and it also enriches the IL toxicity data.

Keywords

EN

Ionic liquid; [BMIM]Cl; toxicity; Saccharomyces cerevisiae AY93161; lignocellulosic ethanol production

• Publisher: De Gruyter Open
• Journal: Polish Journal of Chemical Technology
• Year: 2013
• Volume: 15
• Issue: 2
• Pages: 94-98

Dates

published

1 - 07 - 2013

online

10 - 07 - 2013

Contributors

author

Shengdong Zhu

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

author

Pei Yu

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

author

Mingke Lei

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

author

Yanjie Tong

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

author

Lu Zheng

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

author

Rui Zhang

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

author

Jun Ji

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

author

Qiming Chen

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

author

Yuanxin Wu

• School of Chemical Engineering and Pharmacy, Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Chemical Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan 430073, People’s Republic of China

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Identifiers

DOI

10.2478/pjct-2013-0029