Milk-derived angiotensin-I-converting enzymeinhibitory peptides generated by Lactobacillus delbrueckii subsp. lactis CRL 581

• Authors: Josefina M. Villegas , Gianluca Picariello , Gianfranco Mamone , Maria Beatriz Espeche Turbay , Graciela Savoy de Giori , Elvira Maria Hebert
• Languages of publication: EN

Abstracts

EN

Several strains of Lactobacillus helveticus and Lactobacillus delbrueckii subsp. lactis were evaluated for their ability to release angiotensin-I-converting enzyme (ACE) inhibitory peptides from α-casein (α-CN) and β-casein (β-CN). Casein peptides resulting from L. delbrueckii subsp. lactis CRL 581-mediated hydrolysis exhibited the highest ACE-inhibitory (ACEI) activities, with values of 53 and 40% for α-CN and β-CN, respectively. The casein hydrolysates were fractionated by reversedphase high pressure liquid chromatography and some of the active peptides were identified by mass spectrometry. The fraction with the highest ACEI activity arose from β-CN and contained a mixture of the β-CN f194-206 (QEPVLGPVRGPFP) and f198-206 (LGPVRGPFP) peptides. Furthermore, the ACEI tripeptide IPP was identified in all β-CN hydrolysates; L. delbrueckii subsp. lactis CRL 581 produced the highest amount of this peptide. The bioactive peptides released by CRL 581 strain may be used in the formulation of functional foods and nutraceuticals, representing a healthier and natural alternative for regulating blood pressure.

Keywords

EN

lactic acid bacteria; proteinase; ACE-inhibitory peptides; casein

• Publisher: De Gruyter Open
• Journal: Peptidomics
• Year: 2014
• Volume: 1
• Issue: 1

Dates

published

1 - 1 - 2014

online

10 - 3 - 2014

received

26 - 7 - 2013

accepted

6 - 12 - 2013

Contributors

author

Josefina M. Villegas

• Centro de Referencia para Lactobacilos (CERELA) CONICETChacabuco 145 – 4000 S. M. de Tucuman –Argentina

author

Gianluca Picariello

• Istituto di Scienze dell‘Alimentazione CNR, Via Roma 64, I-83100 Avellino, Italy

author

Gianfranco Mamone

• Istituto di Scienze dell‘Alimentazione CNR, Via Roma 64, I-83100 Avellino, Italy

author

Maria Beatriz Espeche Turbay

• Centro de Referencia para Lactobacilos (CERELA) CONICETChacabuco 145 - 4000 S. M. de Tucuman -Argentina

author

Graciela Savoy de Giori

• Centro de Referencia para Lactobacilos (CERELA) CONICETChacabuco 145 - 4000 S. M. de Tucuman -Argentina

author

Elvira Maria Hebert

• CERELA-CONICET. Chacabuco 145, 4000 San Miguel de Tucuman, Tucuman, Argentina.

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Identifiers

DOI

10.2478/ped-2014-0002