Molecularly imprinted microspheres prepared by precipitation polymerization at high monomer concentrations

• Authors: Tibor Renkecz , Krisztina László , Viola Horváth
• Languages of publication: EN

Abstracts

EN

Highly crosslinked polymer microparticles have been prepared by precipitation polymerization using high monomer loadings (≥25 v/v %) which generally would lead to bulk monoliths. The microparticle format was achieved by the use of non-solvating diluents either alone or in combination with co-solvents. Two distinct morphologies were observed. Monodisperse smooth microspheres were obtained using a thermodynamically good co-solvent whereas segmented irregular particles were formed with poorer co-solvents. It has been found that during polymerization the forming polymer particles were enriched in the co-solvent and this effect was more pronounced when good co-solvents were used. The type of functional monomer, crosslinker and co-solvent, and the non-solvent/co-solvent ratio were identified as influential parameters on the microparticle morphology. With the proposed methodology molecularly imprinted microparticles have been prepared successfully for three different templates, naproxen, diclofenac and toltrazuril using various functional monomers, crosslinkers and polymerization solvent mixtures.

Keywords

EN

molecularly imprinted microparticle; monodisperse; microsphere; particle morphology; nonsolvent

• Publisher: De Gruyter Open
• Journal: Molecular Imprinting
• Year: 2014
• Volume: 2
• Issue: 1

Dates

published

1 - 1 - 2014

received

10 - 3 - 2014

online

21 - 5 - 2014

accepted

23 - 4 - 2014

Contributors

author

Tibor Renkecz

• Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4., H-1111, Budapest, Hungary

author

Krisztina László

• Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, Műegyetem rkp. 3., H-1111, Budapest, Hungary

author

Viola Horváth

• MTA-BME Research Group of Technical Analytical Chemistry, Szt. Gellért tér 4., H-1111, Budapest, Hungary

References

• [1] Huck C.W., Bonn G.K., Poly(styrene-divinylbenzene) based media for liquid chromatography, Chem. Eng. Technol., 2005, 28, 1457-1472.[Crossref]
• [2] Benes M.J., Horak D., Svec F., Methacrylate-based chromatographic media, J. Sep. Sci., 2005, 28, 1855-1875.[Crossref]
• [3] Kawaguchi H., Functional polymer microspheres, Prog. Polym. Sci., 2000, 25, 1171-1210.[Crossref]
• [4] Saralidze K., Koole L.H., Knetsch M.L.W., Polymeric Microspheres for Medical Applications, Materials, 2010, 3, 3537-3564.
• [5] Renkecz T., Horvath V., Horvai G., Molecularly imprinted polymers for chromatography and related techniques, In: Alvarez-Lorenzo, C., Concheiro, A. (Eds.), Handbook of molecularly imprinted polymers, 1st ed., Smithers Rapra, Shrewsbury, 2013.
• [6] Carro-Diaz A.M., Lorenzo-Ferreira R.A., Molecularly imprinted polymers for sample preparation, In: Alvarez-Lorenzo, C., Concheiro, A. (Eds.), Handbook of molecularly imprinted polymers, 1st ed., Smithers Rapra Shrewsbury, 2013.
• [7] Baggiani C., Anfossi L., Giovannoli C., MIP-based immunoassays: State of the Art, limitations and Perspectives, Molecular Imprinting, 2013, 1, 41-54.
• [8] Gokmen M.T., Du Prez F.E., Porous polymer particles-A comprehensive guide to synthesis, characterization, functionalization and applications, Prog. Polym. Sci., 2012, 37, 365-405.[Crossref][WoS]
• [9] Haginaka J., Molecularly imprinted polymers as affinity-based separation media for sample preparation, J. Sep. Sci., 2009, 32, 1548-1565.[Crossref]
• [10] Ye L., Cormack P.A.G., Mosbach K., Molecularly imprinted monodisperse microspheres for competitive radioassay, Anal. Commun., 1999, 36, 35-38.[Crossref]
• [11] Li K., Stover H.D.H., Synthesis of monodisperse poly(divibylbenzene) microspheres, J. Polym. Sci. Part A: Polym. Chem., 1993, 31, 3257-3263.[Crossref]
• [12] Chen L.X., Xu S.F., Li J.H., Recent advances in molecular imprinting technology: current status, challenges and highlighted applications, Chem. Soc. Rev., 2011, 40, 2922-2942.[Crossref][WoS]
• [13] Downey J.S., Frank R.S., Li W.H., Stover H.D.H., Growth mechanism of poly(divinylbenzene) microspheres in precipitation polymerization, Macromolecules, 1999, 32, 2838-2844.
• [14] Shim S.E., Yang S., Jin M.J., Chang Y.H., Choe S., Effect of the polymerization parameters on the morphology and spherical particle size of poly(styrene-co-divinylbenzene) prepared by precipitation polymerization, Colloid Polym. Sci., 2004, 283, 41-48.
• [15] Horvath V., Lorantfy B., Toth B., Bognar J., Laszlo K., Horvai G., Preparation of terbutylazine imprinted polymer microspheres using viscous polymerization solvents, J. Sep. Sci., 2009, 32, 3347-3358.[Crossref][WoS]
• [16] Renkecz T., Laszlo K., Horvath V., In situ synthesis of molecularly imprinted nanoparticles in porous support membranes using high-viscosity polymerization solvents, J. Mol. Recognit., 2012, 25, 320-329.[Crossref][WoS]
• [17] Hansen C.M., Hansen Solubility Paramers: A User‘s Handbook, 2nd ed., CRC Press, Boca Raton, 2007.
• [18] Bandrup J., Immergut E.H., Grulke E.A., Polymer Handbook, 4th ed., Wiley-Interscience, New York, 1999.
• [19] Sellergren B., Shea K.J., Influence of polymer morphology on the ability of imprinted network polymers to resolve enantiomers, J. Chromatogr., 1993, 635, 31-49.
• [20] Sherrington D.C., Preparation, structure and morphology of polymer supports, Chem. Commun., 1998, 2275-2286.[Crossref]

Identifiers

DOI

10.2478/molim-2014-0001