Pressurized hot water extraction coupled to molecularly
imprinted polymers for simultaneous extraction and clean-up
of pesticides residues in edible and medicinal plants of the
Okavango Delta, Botswana

• Authors: Janes Mokgadi , Shima Batlokwa , Keta Mosepele , Veronica Obuseng , Nelson Torto
• Languages of publication: EN

Abstracts

EN

In this study, an in-cell extraction and clean-up approach, employing
pressurized hot water extraction (PHWE) coupled to a molecularly imprinted
polymer (MIP) is proposed. The selectivity of PHWE was improved
through the use of a chlorophyll MIP (PHWE-MIP) for the determination of
organochlorine pesticides residue levels in various edible and medicinal
plants of the Okavango Delta, Botswana. The PHWE-MIP method achieved
simultaneous extraction and clean-up. PHWE employed an optimal
temperature of 260 °C, pressure of 90 bar and flow rate of 1 mL min-1 in 10 min for the extraction of the pesticides from plants while the MIP
selectively overcame the interfering chlorophyll prior to analysis with gas
chromatograph coupled to electron capture detector or mass spectrometer
(GC-ECD/MS). The results obtained were compared to the QuEChERS
Official Method 2007:01 for pesticides residue analysis. The proposed
method seems to be nearly fully automated, environmental friendly,
selective, simple and quick. Moreover, the recoveries of planar pesticides
were improved (93-95%) with relative standard deviations (%RSD) of less
than 10%.

Keywords

EN

Subcritical water extraction ; Molecular imprinted polymers ; In-cell clean up; Sample preparation ; Environmentally friendly

• Publisher: De Gruyter Open
• Journal: Molecular Imprinting
• Year: 2012
• Volume: 1
• Pages: 55-64

Dates

online

08 - 10 - 2013

received

15 - 03 - 2013

accepted

28 - 08 - 2013

Contributors

author

Janes Mokgadi

• Department of Chemistry,
  Rhodes University, P O Box 94,
  Grahamstown, 6140, South Africa

author

Shima Batlokwa

• Department of Earth & Environmental
  Science (Chemistry Unit), Botswana
  International University of Science
  & Technology, P.O. Box 041, Bontleng,
  Gaborone, Botswana

author

Keta Mosepele

• University of Botswana - Okavango
  Research Institute, Private Bag 85,
  Maun, Botswana

author

Veronica Obuseng

• Department of Chemistry,
  University of Botswana,
  Private Bag 00704, Gaborone,
  Botswana

author

Nelson Torto

• Department of Chemistry,
  Rhodes University, P O Box 94,
  Grahamstown, 6140, South Africa

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Identifiers

DOI

10.2478/molim-2013-0003