Hydrolysis of Lactose from Cheese Whey Using a Reactor with β-Galactosidase Enzyme Immobilised on a Commercial UF Membrane

• Authors: Silvina A. Regenhardt , Enrique J. Mammarella , Amelia C. Rubiolo
• Languages of publication: EN

Abstracts

EN

In this study, β-galactosidase enzyme from Kluyveromyces fragilis was immobilised on a commercial polyethersulfone membrane surface, 10 kDa cut-off. An integrated process, concerning the simultaneous hydrolysis-ultrafiltration of whey lactose was studied and working conditions have been fixed at 55°C and pH 6.9, the same conditions that are used for the industrial process of protein concentration. For the immobilisation, best results were obtained using 5% (v/v) of glutaraldehyde solution and 0.03 M galactose; the total activity recovery coefficient (TARC) was 44.2%. The amount of immobilised enzyme was 12.49 mg with a total activity of 86.3 LAU at 37°C, using 5% (w/v) lactose solution in phosphate buffer (100 mM pH 6.9). The stability of the immobilised enzyme was approximately 585 fold higher in comparison with the stability of free enzyme. Multipoint covalent immobilisation improves the stability of the enzyme, thereby enhancing the decision to use the membrane as a filtering element and support for the enzyme immobilisation.

Keywords

EN

membrane bioreactor; immobilised enzyme; β-galactosidase; lactose hydrolysis

• Publisher: De Gruyter Open
• Journal: Chemical and Process Engineering
• Year: 2013
• Volume: 34
• Issue: 3
• Pages: 375-385

Dates

published

1 - 09 - 2013

online

08 - 10 - 2013

Contributors

author

Silvina A. Regenhardt

• Instituto de Desarrollo Tecnológico para la Industria Química – INTEC (Universidad Nacional del Litoral – CONICET). Güemes 3450, (S3000GLN) Santa Fe, Argentina

author

Enrique J. Mammarella

• Instituto de Desarrollo Tecnológico para la Industria Química – INTEC (Universidad Nacional del Litoral – CONICET). Güemes 3450, (S3000GLN) Santa Fe, Argentina

author

Amelia C. Rubiolo

• Instituto de Desarrollo Tecnológico para la Industria Química – INTEC (Universidad Nacional del Litoral – CONICET). Güemes 3450, (S3000GLN) Santa Fe, Argentina

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Identifiers

DOI

10.2478/cpe-2013-0030