Nitrate reductase activity in chosen mosses: a case of study in skalny colliery waste tip / Aktywność reduktazy azotanowej u wybranych mchów na przykładzie hałdy powęglowej skalny

• Authors: Marek Krywult , Anna Salachna , Damian Chmura , Jan Żarnowiec
• Languages of publication: EN

Abstracts

EN

Two species of mosses in relation to nitrogen metabolism were examined. This subject is little known in this group of plant. Investigations of nitrate reductase activity in green tissues of Brachythecium rutabulum (Hedw.) Schimp. and Atrichum undulatum (Hedw.) P.Beauv. were performed. The study was conducted in two localities: heavy contaminated waste tip Skalny located in Upper Silesia, and Blonia City Park in Bielsko-Biala which place was chosen as a control area. For both species high activity of the enzyme was detected. The nitrate reductase activity varied between 99 to 9093 nmol per g dry mass per hour for B. rutabulum and 265 to 5135 nmol per g d.m. per hour of nitrite synthesized for A. undulatum respectively on Skalny waste tip. In the control area the results varied between 747 to 1077 for B. rutabulum and 171 to 518 nmol per g d.m. per hour of nitrite synthesized for A. undulatum, respectively. The differences were statistically significant only between the two species but not between habitats probably due to high dispersion and small amount of replications. The levels of nitrate and nitrite in stream water in both areas were also measured. In the Skalny waste tip there were high and reached 1.66 mg · dm-3 of nitrite and 65 mg · dm-3 of nitrate, respectively. In the control area these amounts were lower and reach zero level for nitrite and 4.5 mg · dm-3 of nitrate, respectively.

PL

Dwa gatunki mchów badano pod kątem asymilacji azotu. To zagadnienie jest mało poznane u tej grupy roślin. Przeprowadzono badania aktywności reduktazy azotanowej w zielonych tkankach Brachythecium rutabulum (Hedw.) Schimp. i Atrichum undulatum (Hedw.) P.Beauv. Badania zostały wykonane w dwóch miejscach: na hałdzie powęglowej Skalny na Górnym Śląsku i w parku w dzielnicy Błonia w Bielsku-Białej, które zostało wybrane jako miejsce kontrolne. Dla obu gatunków stwierdzono wysoką aktywność enzymu. Aktywność reduktazy azotanowej wahała się od 99 do 9093 nmol na g suchej masy na godzinę dla B. rutabulum i 265 do 5135 nmol na g suchej masy na godzinę azotynu syntetyzowanego u A. undulatum na hałdzie Skalny. W miejscu kontrolnym wyniki wahały się od 747 do 1077 dla B. rutabulum i 171 do 518 nmol na g sm/h u A. undulatum. Różnice były statystycznie znaczące tylko pomiędzy gatunkami, ale nie pomiędzy typem siedliska prawdopodobnie ze względu na wysoką dyspersję i małą liczbę powtórzeń. Zmierzono także poziom azotanów i azotynów w strumieniu wody w obu miejscach. Na hałdzie Skalny zawartość była wysoka i osiągnęła odpowiednio: 1,66 mg dm-3 azotynu oraz 65 mg dm-3 azotanu. W miejscu kontrolnym wartości te były niższe i osiągnęły odpowiednio poziom zerowy dla azotynów i 4,5 mg dm-3 dla azotanu.

Keywords

EN

bryophytes; nitrogen fixation; environmental pollution

• Publisher: De Gruyter Open
• Journal: Chemistry-Didactics-Ecology-Metrology
• Year: 2013
• Volume: 18
• Issue: 1-2
• Pages: 61-68

Dates

published

1 - 12 - 2013

online

22 - 01 - 2014

Contributors

author

Marek Krywult

• Institute of Environmental Protection and Engineering, University of Bielsko-Biala, ul. Willowa 2, 43-309 Bielsko-Biała, Poland

author

Anna Salachna

• Institute of Environmental Protection and Engineering, University of Bielsko-Biala, ul. Willowa 2, 43-309 Bielsko-Biała, Poland

author

Damian Chmura

• Institute of Environmental Protection and Engineering, University of Bielsko-Biala, ul. Willowa 2, 43-309 Bielsko-Biała, Poland

author

Jan Żarnowiec

• Institute of Environmental Protection and Engineering, University of Bielsko-Biala, ul. Willowa 2, 43-309 Bielsko-Biała, Poland

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Identifiers

DOI

10.2478/cdem-2013-0018