A study of sample mineralization methods for arsenic analysis of blood and urine by hydride generation and graphite furnace atomic absorption spectrometry

• Authors: Jirina Sysalova , Vera Spevackova
• Languages of publication: EN

Abstracts

EN

Mineralization procedures for blood and urine suitable for the determination of arsenic by Hydride Generation Atomic Absorption Spectrometry (HGAAS) are studied on model samples, and the results are utilized in biological monitoring investigations. The objective of this work is to obtain good total As recoveries for both matrices regardless of added As species (As(III), As(V), DMA, MMA, AsB, or AsC). Prior to the HGAAS analyses, preparation procedures were controlled under optimised conditions by graphite furnace atomic absorption spectrometry (GFAAS). Two preparation procedures for urine give As recoveries close to 100% by HGAAS: a) dry ashing at 420°C with Mg(NO3)2 on a hot plate, and b) microwave oven decomposition with (NH4)2S2O8. For blood samples, As recoveries by HGAAS range between 95 and 108% for all species when using dry ashing after a pretreatment of samples with HNO3 and H2O2 in a microwave oven. Wet digestion with (NH4)2S2O8 in a microwave oven gives recoveries very near 100% for Asinorg. and MMA. For other As species in spiked blood samples, recoveries of less than 20% As are found. Precision and detection limits obtained by both techniques are evaluated as well. For arsenic concentrations of 20 μg dm−3 or more in blood and urine, a chemical modifier is recommended for GFAAS analysis; it may or may not be proceeded by a mineralization step. For low As levels encountered in the unexposed population, the HGAAS technique provides reliable results only if a very complete mineralization procedure is used.

Keywords

EN

Arsenic; Blood; Urine; Sample preparation; HGAAS; GFAAS

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2003
• Volume: 1
• Issue: 2
• Pages: 108-120

Dates

published

1 - 6 - 2003

online

1 - 6 - 2003

Contributors

author

Jirina Sysalova

• Institute of Analytical Chemistry, Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czech Republic

author

Vera Spevackova

• National Institute of Public Health, Srobarova 48, 100 42, Prague 10, Czech Republic

References

• [1] R. Kubínová: “System of Monitoring the Environmental Impact on Population Health in the Czech Republic 1994”, Project V, Editor National Institute of Public Health, Prague, Czech Republic, ISBN 80-900075-1-1, 1995.
• [2] E. Hakala and L. Pyy: “Selective determination of toxicologically important arsenic species in urine by high-performance liquid chromatography-hydride generation atomic absorption spectrometry”, J. Anal. At. Spectrom., Vol. 7, (1992), pp. 191–196. http://dx.doi.org/10.1039/ja9920700191[Crossref]
• [3] M. Jakubovski, M. Trzcinka-Ochocka, G. Razniewska, W. Matczak: “Biological monitoring in occupational exposure to arsenic by determining urinary content of inorganic arsenic and its methylated metabolites”, Int. Arch. Occup. Environ. Health, Vol. 71, (1998), pp. 29–32. http://dx.doi.org/10.1007/s004200050246[Crossref]
• [4] M. Vahter, L. Friiberg, B. Rahnster, A. Nygren, P. Nolinder: “Airborne arsenic and urinary excretion of metabolites of inorganic arsenic among smelter workers”, Int. Arch. Occup. Environ. Health, Vol. 57, (1986), pp. 79–91. http://dx.doi.org/10.1007/BF00381375[Crossref]
• [5] M. Buratti, G. Calzaferri, G. Caravelli, A. Colombi, M. Maroni, V. Foa: “Significance of arsenic metabolic forms in urine”, Int. J. Eviron. Anal. Chem, Vol. 17, (1984), pp. 25–34. [Crossref]
• [6] M.A. López-Gonzáles, M. Gómez, C. Cámara, M.A. Palacios: “Determination of toxic and non-toxic arsenic species in urine by microwave assisted mineralization and hydride generation atomic absorption spectrometry”, Mikrochim. Acta, Vol. 120, (1995), pp. 301–308. http://dx.doi.org/10.1007/BF01244440[Crossref]
• [7] WHO IPCS: “Arsenic”, International Programme on Chemical Safety, Environmental Health Criteria 18, WHO Geneva, 1981, ISBN 92 4 154078 8.
• [8] J.P. Buchet, J. Pauwels, R. Lauwerys: “Assessment of exposure to inorganic arsenic following ingestion of marine organisms by volunteers”, Environ. Res., Vol. 66, (1994), pp. 44–51. http://dx.doi.org/10.1006/enrs.1994.1043[Crossref]
• [9] D.K. Eaton and J.R. McCutcheon: “Matrix modification for furnace atomic aborption spectrometric determination of arsenic in whole human blood”, J. Anal. Toxicol., Vol. 9, (1985), pp. 213–216. [Crossref]
• [10] L. Fishbein: “Perspectives of analysis of carcinogenic and mutagenic metals in biological samples”, Int. J. Environ. Anal. Chem., Vol. 28, (1987), pp. 21–69. [Crossref]
• [11] D.E. Nixon, G.V. Mussmann, S.J. Eckdahl, T.P. Moyer: “Total arsenic in urine: Palladium-persulfate vs nickel as a matrix modifier for graphite furnace atomic absorption spectrometry”, Clin. Chem., Vol. 37, (1991), pp. 1575–1579.
• [12] L. Pszonicki and J. Dudek: “Modifier effects in the determination of arsenic, antimony and bismuth by electrothermal atomic absorption spectrometry”, J. Anal. At. Spectrom., Vol. 14, (1999), pp. 1755–1760. http://dx.doi.org/10.1039/a905984i[Crossref]
• [13] J. Kučera and L. Soukal: “Determination of As, Cd, Cu, Hg, Mo, Sb, and Se in biological reference materials by radiochemical neutron activayion analysis”, J. Radioanal. Nucl. Chem., Vol. 168, (1993), pp. 185–199. http://dx.doi.org/10.1007/BF02040891[Crossref]
• [14] G.M. Momplaisir, J.-S. Blais, M. Quinteiro, W.D. Marshall: “Determination of arsenobetaine, arsenocholine, and tetramethylarsonium cations in seafoods and human urine by high-performance liquid chromatography-termochemical hydride generation-atomic absorption spectrometry”, J. Agric. Food Chem., Vol. 39, (1991), pp. 1448–1451. http://dx.doi.org/10.1021/jf00008a017[Crossref]
• [15] V. Spěváčková, M. Čejchanová, J. Sysalová, P. Šubrt, J Mareček: “Determination of arsenic in human fluids for monitoring purposes”, Programm Vortragskurzfassungen CANAS'99 Konstanz, March 14–19, 1999, p. 148.
• [16] J. Sysalová, V. Spěváčková, M. Čejchanová: “Method development for arsenic determination in biological monitoring to environmental exposure”, Contemporary state, development and applications of spectroscopic methods, Proceedings of the 4th European Furnace Symposium and XVth Slovak Spectroscopic Conference, Podbanské (SK), June 12–16, 2000, p. 181.
• [17] N. Ybánez, M.L. Cervera, R. Montoro: “Comparison of dry mineralization and microwave-oven digestion for the determination of arsenic in mussel products by platform in furnace Zeeman-effect atomic absorption spectrometry”, J. Anal. At. Spectrom., Vol. 6, (1991), pp. 379–384. http://dx.doi.org/10.1039/ja9910600379[Crossref]
• [18] E.H. Larsen: “Electrothermal atomic absorption spectrometry of inorganic and organic arsenic species using conventional and fast furnace programmes”, J. Anal. At. Spectrom., Vol. 6, (1991), pp. 375–377. http://dx.doi.org/10.1039/ja9910600375[Crossref]
• [19] T. Guo, J. Baasner, D.L. Tsalev: “Fast automates determination of toxicologically relevant arsenic in urine by flow injection hydride generation atomic absorption spectrometry”, Anal. Chim. Acta, Vol. 349, (1997), pp. 313–318. http://dx.doi.org/10.1016/S0003-2670(97)00276-6[Crossref]

Identifiers

DOI

10.2478/BF02479263