Structural identification of the novel
3 way-junction motif

• Authors: Dominika Jedrzejczyk , Arkadiusz Chworos
• Languages of publication: EN

Abstracts

EN

A novel RNA motif was identified based on its
sequence by computational structure modeling. The RNA
molecule was reported to be a substrate for the structurally
specific endoribonuclease, Dicer, which cleaves doublestranded
RNA and cuts out 20−25 nucleotide fragments.
This enzymatic property was essential for the potential
utilization of the motif in the nanoparticle design of
further biological experiments. Herein, the protocol for the
prediction of the structure of this motif in-silico is presented,
starting from its primary sequence and proceeding
through secondary and tertiary structure predictions.
Applying RNA architectonics, this novel structural motif,
3wj-nRA, was used for rational RNA nanoparticle design.
The molecules, which are based on this three-way junction
fold, may assemble into more complex, triangular shaped
nano-objects. This trimeric nanoparticle containing
3wj-nRA motif can be further utilized for functionalization
and application.

Keywords

EN

molecular modeling; computationalbiochemistry; structural RNA biology; RNA nanoparticles; RNA architectonics

• Publisher: De Gruyter Open
• Journal: DNA and RNA Nanotechnology
• Year: 2015
• Volume: 2
• Issue: 1

Dates

online

18 - 12 - 2015

received

26 - 7 - 2015

accepted

30 - 9 - 2015

Contributors

author

Dominika Jedrzejczyk

author

Arkadiusz Chworos

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Identifiers

DOI

10.1515/rnan-2015-0004