Functional investigation of Bacillus subtilis YrkF’s
involvement in sulfur transfer reactions

• Authors: Hannah L. Martin , Katherine A. Black , Patricia C. Dos Santos
• Languages of publication: EN

Abstracts

EN

Sulfur incorporation into the molybdenum
cofactor (Moco) in the Gram-negative bacterium
Escherichia coli involves six enzymes. The initial
reaction includes the cysteine desulfurase IscS, the
sulfurtransferase TusA, and the rhodanese domaincontaining
protein YnjE. The Gram-positive bacterium
Bacillus subtilis contains no direct homologs for IscS,
but rather four distinct cysteine desulfurases (YrvO, NifS,
NifZ, SufS) and YrkF, a two-domain rhodanese protein
with an N-terminal domain similar to TusA. Bioinformatic
analysis was used to identify potential enzymes involved
in the B. subtilis Moco thiolation pathway and in vitro
reactions demonstrated that YrkF can accept sulfur from
and enhance the activity of YrvO.

Keywords

EN

cysteine desulfurase; YrvO; YrkF; Moco; Bacillus subtilis; persulfide; sulfurtransferase

• Publisher: De Gruyter Open
• Journal: Peptidomics
• Year: 2015
• Volume: 2
• Issue: 1

Dates

online

20 - 1 - 2016

accepted

5 - 12 - 2015

received

6 - 8 - 2015

Contributors

author

Hannah L. Martin

• Department of Chemistry,
  Wake Forest University

author

Katherine A. Black

• Department of Chemistry,
  Wake Forest University

author

Patricia C. Dos Santos

• Wake Forest University,
  1834 Wake Forest Road, Winston Salem, North Carolina, 27106

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Identifiers

DOI

10.1515/ped-2015-0008