Peptidomics in veterinary science: focus on bovine paratuberculosis

• Authors: Cristian Piras , Alessio Soggiu , Viviana Greco , Isabella Alloggio , Luigi Bonizzi , Paola Roncada
• Languages of publication: EN

Abstracts

EN

Bacterial infections represent a serious burden
both for animal production and human health (zoonosis).
Faster and more reliable diagnosis are mandatory in order
to avoid economic losses and antibiotics misuse. The
development of new potential diagnostic strategies for
the immunodetection of pathogens is closely linked to
the discovery of small polypeptides with immunogenic
or immunoreactive activity. The candidate peptides used
for this purpose must have several properties principally
represented by their specificity and their location in
the bacterial cell. Both proteomics, peptidomics and
bioinformatics represent powerful complementary tools
to discover specific immunoreactive peptides useful for
diagnosis or vaccine. Peptidomics of Mycobacterium
avium subsp. paratuberculosis (MAP) represents a good
example of the potential of this discovery-phase. This
review reports a comprehensive update of the current
scientific knowledge about proteins and peptides of
MAP with already documented humoral response. These
findings, together with bioinformatics tools available,
could be extremely useful to design a better strategy
for subclinical bovine paratuberculosis diagnosis. The
knowledge provided also represents a reliable example
on the workflow to be followed in the direction of
the diagnosis of other diseases through a peptidomic
approach.

Keywords

EN

immunoreactive peptides; bioinformatics; bovine paratuberculosis; diagnostic peptides

• Publisher: De Gruyter Open
• Journal: Peptidomics
• Year: 2015
• Volume: 2
• Issue: 1

Dates

received

3 - 10 - 2014

accepted

30 - 11 - 2014

online

30 - 3 - 2015

Contributors

author

Cristian Piras

• Dipartimento di Scienze
  Veterinarie e Sanità Pubblica, Università degli studi di Milano,
  Milano, Italy

author

Alessio Soggiu

• Dipartimento di Scienze
  Veterinarie e Sanità Pubblica, Università degli studi di Milano,
  Milano, Italy

author

Viviana Greco

• Fondazione Santa Lucia – IRCCS, Rome, Italy
• Dipartimento di medicina sperimentale e chirurgia,
  Università degli studi di Roma TorVergata, Roma, Italy

author

Isabella Alloggio

• Dipartimento di Scienze
  Veterinarie e Sanità Pubblica, Università degli studi di Milano,
  Milano, Italy

author

Luigi Bonizzi

• Dipartimento di Scienze
  Veterinarie e Sanità Pubblica, Università degli studi di Milano,
  Milano, Italy

author

Paola Roncada

• Dipartimento di Scienze
  Veterinarie e Sanità Pubblica, Università degli studi di Milano,
  Milano, Italy
• Istituto Sperimentale Italiano L. Spallanzani, Milano,
  Ital

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Identifiers

DOI

10.1515/ped-2015-0001