Attempt to assess the infiltration of enamel made
with experimental preparation using a scanning
electron microscope

• Authors: Małgorzata Skucha-Nowak
• Languages of publication: EN

Abstracts

EN

Introduction: The resin infiltration technique,
a minimally invasive method, involves the saturation,
strengthening, and stabilization of demineralized enamel
by a mixture of polymer resins without the need to use
rotary tools or the risk of losing healthy tooth structures. Aim of the study: 1. To design and synthesize an experimental infiltrant
with potential bacteriostatic properties. 2. To compare the depth of infiltration of the designed
experimental preparation with the infiltrant available
in the market using a scanning electron microscope. Material and Methods: Composition of the experimental
infiltrant was established after analysis of 1H NMR
spectra of the commercially available compounds that
can penetrate pores of demineralized enamel. As the infiltrant
should have bacteriostatic features by definition,
an addition of 1% of monomer containing metronidazole
was made. Thirty extracted human teeth were soaked in
an acidic solution, which was to provide appropriate conditions
for demineralization of enamel. Afterward, each
tooth was divided along the coronal-root axis into two
zones. One zone had experimental preparation applied to
it (the test group), while the other had commercially available
Icon (the control group). The teeth were dissected
along the long axis and described above underwent initial
observation with use of a Hitachi S-4200 scanning electron
microscope. Results: It was found that all samples contained only
oxygen and carbon, regardless of the concentration of
additions introduced into them. The occurrence of carbon
is partially because it is a component of the preparation in question and partially because of sputtering of the sample
with it. Hydrogen is also a component of the preparation,
as a result of its phase composition; however, it cannot be
detected by the EDS method. Conclusions: 1. SEM, in combination with X-ray microanalysis, does
not allow one to explicitly assess the depth of penetration
of infiltration preparations into enamel. 2. In order to assess the depth of penetration of infiltration
preparations with use of X-ray microanalysis, it
is recommended to introduce a contrast agent that is
approved for use in dental materials, such as ytterbium
III fluoride.

Keywords

EN

Infiltrants; enamel; caries detection/diagnosis; minimally invasive dentistry; scanning electronmicroscope; preventive dentistry.

• Publisher: De Gruyter Open
• Journal: Open Medicine
• Year: 2014
• Volume: 10
• Issue: 1

Dates

accepted

18 - 3 - 2015

received

29 - 12 - 2014

online

3 - 4 - 2015

Contributors

author

Małgorzata Skucha-Nowak

• Department
  of Conservative Dentistry with Endodontics, Medical University of
  Silesia in Katowice, Plac Akademicki 17, 41-902 Bytom

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Identifiers

DOI

10.1515/med-2015-0036