Results found: 3

Number of results on page

Search results

Sort By:

Limit search:

How "fast" reading of prenatal cardiac diagnosis may badly influence the neonatal management - case report from tertiary center in 2015 and literature review

100%

Piątek K., Zych-Krekora K., Płużańska J., Gulczyńska E., Respondek-Liberska M.

issue 3

38-41

Complains about prenatal diagnoses usually touch late diagnosis, missed diagnosis or uncomplete diagnosis. Prenatal diagnose provides usually important information for parents, obstetrician and neonatologist. Successful perinatal care is based on a good cooperation of the perinatal team. This time we present a peculiar situation when improper reading of prenatal diagnosis had caused a lot of troubles for the patient, parents and hospital staff.

Overexpression of genes involved in phytochelatin biosynthesis in Escherichia coli: effects on growth, cadmium accumulation and thiol level.

86%

Wawrzyńska A., Wawrzyński A., Gaganidze D., Kopera E., Piątek K., Bal W., Sirko A.

2005

vol. 52

issue 1

109-116

In Escherichia coli, heterologous production of Schizosaccharomyces pombe phytochelatin synthase (PCS) along with overproduction of E. coli serine acetyltransferase (SAT) and γ-glutamylcysteine synthase (γECS) was achieved and resulted in the accumulation of phytochelatins in bacterial cells. Overproduction of either γECS alone or simultaneous production of all three proteins in bacterial cells were accompanied by reduced growth rate in liquid cultures. Interestingly, bacteria overproducing either γECS or both SAT and γECS (with elevated level of γ-glutamylcysteine but not of phytochelatins) were able to accumulate more cadmium per dry weight than the control. However, the most efficient cadmium accumulation was observed in bacteria with elevated levels of all three proteins: SAT, γECS and PCS. Therefore, "pushing" the entire pathway might be the most promising approach in modification of bacteria for potential bioremediation purposes because the level of intermediates, cysteine and glutathione, can limit the rate of production of phytochelatins. However, in such bacteria other metabolic process might become limiting for efficient growth.

Alternative 3' acceptor site in the exon 2 of human PAX8 gene resulting in the expression of unknown mRNA variant found in thyroid hemiagenesis and some types of cancers

73%

Szczepanek-Parulska E., Szaflarski W., Piątek K., Budny B., Jaszczyńska-Nowinka K., Biczysko M., Wierzbicki T., Skrobisz J., Zabel M., Ruchała M.

2013

vol. 60

issue 4

573-578

PAX8 gene encodes one of the transcription factors engaged in the regulation of proper development of thyroid gland as well as Müllerian and renal/upper urinary tracts. So far, six alternatively spliced transcripts were reported, however, sequences of only four were deposited in the NCBI database. Here, we evaluate a fragment of a novel variant of PAX8 mRNA formed by an alternative 3' acceptor site located in the second exon. The molecular outcome encompasses extension of the 5' untranslated region of exon two by 97 nucleotides as is evident from mRNA. This new insert may impair binding of mRNA to the ribosome and in consequence significantly decrease expression of the PAX8 protein. Here, we show for the first time that the novel insert in exon two might be associated with congenital thyroid hemiagenesis and influence development of different types of cancer.

Refine search results

2 Acta Biochimica Polonica

1 Prenatal Cardiology

1 2015

1 2013

1 2005

3 Piątek K.

1 Bal W.

1 Biczysko M.

1 Budny B.

1 Gaganidze D.

1 Gulczyńska E.

1 Jaszczyńska-Nowinka K.

1 Kopera E.

1 Płużańska J.

1 Respondek-Liberska M.

1 Ruchała M.

1 Sirko A.

1 Skrobisz J.

1 Szaflarski W.

1 Szczepanek-Parulska E.

1 Wawrzyńska A.

1 Wawrzyński A.

1 Wierzbicki T.

1 Zabel M.

1 Zych-Krekora K.

Search results

How "fast" reading of prenatal cardiac diagnosis may badly influence the neonatal management - case report from tertiary center in 2015 and literature review

Overexpression of genes involved in phytochelatin biosynthesis in Escherichia coli: effects on growth, cadmium accumulation and thiol level.

Alternative 3' acceptor site in the exon 2 of human PAX8 gene resulting in the expression of unknown mRNA variant found in thyroid hemiagenesis and some types of cancers