Search results

Search:
in the keywords: yeast two-hybrid

Sort By:

Limit search:

Synaptic protein UNC-13 interacts with an F-box protein that may target it for degradation by proteasomes

100%

Polinsky C., Houston C., Vado J., Shaikh A., Kohn R. E.

2006

vol. 53

issue 1

145-148

UNC-13 protein participates in regulating neurotransmitter release. In Drosophila melanogaster, proteasomal degradation controls UNC-13 levels at synapses. Function of the amino-terminal region of a 207 kDa form of Caenorhabditis elegans UNC-13 is unknown. Yeast two-hybrid and secondary yeast assays identified an F-box protein that interacts with this amino-terminal region. As F-box proteins bind proteins targeted for proteasomal degradation, this protein may participate in degrading a subset of UNC-13 proteins, suggesting that different forms of UNC-13 are regulated differently. Yeast assays also identified an exonuclease, a predicted splicing factor, and a protein with coiled-coil domains, indicating that UNC-13 may affect RNA function.

Vps41, a protein involved in lysosomal trafficking, interacts with caspase-8

88%

Wang L., Pan X., He L., Zhang R., Chen W., Zhang J., Lu M., Hua Z.-C.

2013

vol. 60

issue 1

37-42

Caspase-8 is a member of the cysteine-aspartic acid protease (caspase) family which plays a central role in apoptosis and development. We screened caspase-8 interacting proteins from mouse T-cell lymphoma and 7.5-day embryo cDNA libraries by yeast two-hybrid system and obtained eleven positive clones, including Vacuolar protein sorting 41 (Vps41), a protein involved in trafficking of proteins from the late Golgi to the vacuole. The interaction of Vps41 with caspase-8 was confirmed by co-immunoprecipitation (co-IP) and co-localization studies in HEK293T cells. Co-IP experiments also showed that Vps41 binds to the p18 subunit of caspase-8 through its WD40 region and RING-finger motif. Furthermore, we found that overexpression of Vps41 promotes Fas-induced apoptosis in A549 human lung adenocarcinoma cells. The cleavage of caspase-3, a caspase-8 downstream effector, was increased when cells were transfected with Vps41-overexpressing plasmid. Together, these results suggest a novel interaction of caspase-8 with Vps41 and provide a potential role of Vps41 beyond lysosomal trafficking.

Refine search results

2 Acta Biochimica Polonica

1 2013

1 2006

1 Chen W.

1 He L.

1 Houston C.

1 Hua Z.-C.

1 Kohn R. E.

1 Lu M.

1 Pan X.

1 Polinsky C.

1 Shaikh A.

1 Vado J.

1 Wang L.

1 Zhang J.

1 Zhang R.

Search results

Synaptic protein UNC-13 interacts with an F-box protein that may target it for degradation by proteasomes

Vps41, a protein involved in lysosomal trafficking, interacts with caspase-8