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Production of functional transgenic mice by DNA pronuclear microinjection.

100%

Auerbach A. B.

vol. 51

issue 1

9-31

Successful experiments involving the production of transgenic mice by pronuclear microinjection are currently limited by low efficiency of random transgene integration into the mouse genome. Furthermore, not all transgenic mice express integrated transgenes, or in other words are effective as functional transgenic mice expressing the desired product of the transgene, thus allowing accomplishment of the ultimate experimental goal - in vivo analysis of the function of the gene or gene network. The purpose of this review is to look at the current state of transgenic technology, utilizing a pronuclear microinjection method as the most accepted way of gene transfer into the mouse genome.

Mice and humans: chromosome engineering and its application to functional genomics.

100%

Klysik J.

2002

vol. 49

issue 3

553-569

Functional modeling of human genes and diseases requires suitable mammalian model organisms. For its genetic malleability, the mouse is likely to continue to play a major role in defining basic genetic traits and complex pathological disorders. Recently, gene targeting techniques have been extended towards developing new engineering strategies for generating extensive lesions and rearrangements in mouse chromosomes. While these advances create new opportunities to address similar aberrations observed in human diseases, they also open new ways of scaling-up mutagensis projects that try to catalogue and annotate cellular functions of mammalian genes.

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2 Acta Biochimica Polonica

1 2004

1 2002

1 Auerbach A. B.

1 Klysik J.

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Production of functional transgenic mice by DNA pronuclear microinjection.

Mice and humans: chromosome engineering and its application to functional genomics.