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Small high density lipoprotein subclasses: some of their physico-chemical properties and stability in solution.

100%

Atmeh R. F., Abd Elrazeq I. O.

2005

vol. 52

issue 2

515-525

Small high density lipoproteins (SHDL) contribute to the protection from atherosclerosis, but detailed information about their properties is not available yet. We isolated four of the smallest HDL subclasses that contain apoA-I alone, the small lipoprotein A-I (SLpAI), by their separation on gradient polyacrylamide gel followed by electroelution. Their physico-chemical properties were calculated from their displacement in non-denaturing gradient polyacrylamide gel under the effect of electrical potential. The properties are: Stokes' radii 2.96-3.56 nm; molecular masses 42-70 kDa; net negative charge 7.2-13.5; surface charge densities 3139-4069 -esu∙˙cm^(-2); surface potentials 10.6-15.7 -mV; coefficients of friction 5.74-6.90 × 10-8 g∙˙s^(-1); and diffusion coefficients 5.76-6.94 7× 10-7 cm^2∙˙s^(-1). We found that these particles were of low stability as they underwent molecular transformation into larger particles on storage. The estimated dimensions of these particles do not support ellipsoidal shape, therefore, the most probable shape is spherical; consequently, their hydrated characteristics were estimated. We conclude that these particles have high values of negative surface charge and diffusion coefficients, and are of low stability. Their small Stokes' radii were similar to each other and they are spherical and highly hydrated.

Design considerations for mesoporous silica nanoparticulate systems in facilitating biomedical applications

84%

Desai D., Karaman D. S., Prabhakar N., Tadayon S., Duchanoy A., Toivola D. M., Rajput S., Näreoja T., Rosenholm J. M.

Mesoporous Biomaterials

2014

vol. 1

issue 1

Mesoporous silica nanoparticles (MSNs) have advanced to the forefront of multifunctional nanoparticulate systems in nanomedicine, owing to this highly fexible materials platform enabling a multitude of design options, often in a modular fashion. Drug delivery ability, detectability via diferent imaging modalities, and stimuliresponsiveness are often combined into one particle system. Very sophisticated and versatile designs along with impressive demonstrations of applicability have been reported to date, but a common ground when it comes to some critical considerations valid for any nanoparticle intended for biomedical purposes is lacking to some degree. In this study, we attempt to take a glance at some of the most crucial aspects of biomedical nanoparticulate design and relate how they apply specifically toMSNs. These considerations include fuorophore labeling and leaching with respect to immobilization to MSNs, the surrounding conditions, carrier biodegradability, and surface coating. Surface modifcation strategies and surface charge tuning are further considered in conjunction to the relative amount of cellular uptake and serum protein adsorption. Cellular internalization routes and biological techniques used to evaluate especially in vitro biobehavior are discussed. Our attempt is hereby to draw attention to some of the most frequently occurring issues to be considered in the design of MSN systems for biomedical applications

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1 Acta Biochimica Polonica

1 Mesoporous Biomaterials

1 2014

1 2005

1 Abd Elrazeq I. O.

1 Atmeh R. F.

1 Desai D.

1 Duchanoy A.

1 Karaman D. S.

1 Näreoja T.

1 Prabhakar N.

1 Rajput S.

1 Rosenholm J. M.

1 Tadayon S.

1 Toivola D. M.

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Small high density lipoprotein subclasses: some of their physico-chemical properties and stability in solution.

Design considerations for mesoporous silica nanoparticulate systems in facilitating biomedical applications